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• W2078751197 abstract "ART is a popular treatment but success rate remains still low. One of the reason may be the inclusion of sperm apoptosis. Magnetic activated cell sorting(MACS) with annexin-V microbeads recognizes externalized phosphatidylserine residues on the surface of apoptotic sperm. The aim of this study is to compare the outcome of ART using non-apoptotic sperm using with Annexin V-MACS and that of usual method. Clinical study. Twenty-three patients were received ovarian stimulation with clomiphene and hMG. Oocytes were retrieved at 32 hrs later after GnRHa spray. Motile sperm were selected by density-gradient centrifugation. One aliquot of sperm suspension was used for fertilization by usual method, the other aliquot by Annexin V-MACS. Oocytes(N=64) in each case were divided into two, 34 oocytes were fertilized with the sperm by usual method (C) and 30 oocytes by Annexin V-MACS(VM). After fertilization and culture, one embryo was transferred in 14 cases(9:C, 5:VM). Another embryos were frozen with vitrification method. Fertilization, cleavage, growth rate and pregnancy rate were compared in two groups. Data were statically analyzed by χ2test. The mean concentration of sperm was 50.1x10[sup6]/ml and motility was 57% before the separation. After the separation, the mean concentration of sperm was 24.8x10[sup6]/ml and motility was 91% in “C”, and that of 41.9x10[sup6]/ml and 92% in “VM”, respectively. Fertilization rate was 88.2% and cleavage rate was 100% and blastocyst development rate was 36.7% in “C”, and that was 90%, 85.2% and 33.3% in “VM”, respectively. Pregnancy rate in transferred cases was 44.4%(C) and 40%(VM), respectively. There were no significant differences between two groups. The annexin V-MACS has the potential to benefit ART for the selection of apoptotic sperm. However, we cannot find any significant advantage in V-MACS procedure from this study. A larger scale prospective randomized study is required to evaluate the value of this technique." @default.
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• W2078751197 date "2013-09-01" @default.
• W2078751197 modified "2023-09-30" @default.
• W2078751197 title "Art outcome of non-apoptotic spermatozoa selected by magnetic-activated cell sorting with annexin V-conjugated microbeads" @default.
• W2078751197 doi "https://doi.org/10.1016/j.fertnstert.2013.07.536" @default.
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