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- W2078751392 abstract "Staminate Cucurbita buds undergoing meiosis are fixed for 12–24 hours in a solution containing 3 parts of 95% alcohol, 1 part of acetocarmine to which iron acetate has been added, and a flake of rusted iron. After fixation the buds are washed in 95% alcohol and stored in 95% alcohol with the iron flake for 5-10 days. A stain containing 10 drops of 45% acetic acid, 10 drops of acetocarmine, and 10 drops of brown storage solution is prepared. A small piece of anther is placed in a drop of stain on a slide. At the moment the anther is macerated, the debris is removed, and when the cells turn grey to dark brown a cover slip is applied. The stain is differentiated by gentle heat and the cover slip is sealed with paraffin." @default.
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- W2078751392 date "1954-01-01" @default.
- W2078751392 modified "2023-09-26" @default.
- W2078751392 title "An Acetocarmine Technic forCucurbita" @default.
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- W2078751392 doi "https://doi.org/10.3109/10520295409115456" @default.
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