Matches in SemOpenAlex for { <https://semopenalex.org/work/W2078751476> ?p ?o ?g. }
Showing items 1 to 94 of
94
with 100 items per page.
- W2078751476 endingPage "9413" @default.
- W2078751476 startingPage "9403" @default.
- W2078751476 abstract "We present here an improved analytical method for the analysis of glycation events in proteins. Nonenzymatic glycation of an IgG2 monoclonal antibody was studied using affinity chromatography, mass spectrometry, and chemical derivatization. Analysis of both forced-degraded and bulk-drug substance (BDS) samples showed the presence of glycated protein. A new peptide mapping procedure, incorporating derivatization using sodium borohydride, allowed the development of a sensitive method for detecting and identifying the sites of modification. When combined with tandem mass spectrometry, peptides glycated by glucose showed dramatically improved MS/MS spectra as compared to underivatized controls. Using these methods we were able to map a number of glycation sites in both forced-degraded and BDS samples that were distributed across both light and heavy chain subdomains. The combination of affinity chromatography, high-resolution mass spectrometry, and a simple derivatization procedure should allow the facile analysis of glycation for other antibody and protein samples." @default.
- W2078751476 created "2016-06-24" @default.
- W2078751476 creator A5062806679 @default.
- W2078751476 creator A5079589390 @default.
- W2078751476 creator A5091399120 @default.
- W2078751476 date "2007-11-07" @default.
- W2078751476 modified "2023-09-30" @default.
- W2078751476 title "Characterization of Nonenzymatic Glycation on a Monoclonal Antibody" @default.
- W2078751476 cites W1978793468 @default.
- W2078751476 cites W2011024907 @default.
- W2078751476 cites W2011643386 @default.
- W2078751476 cites W2013816252 @default.
- W2078751476 cites W2015700034 @default.
- W2078751476 cites W2024251603 @default.
- W2078751476 cites W2027914534 @default.
- W2078751476 cites W2032825198 @default.
- W2078751476 cites W2037667906 @default.
- W2078751476 cites W2058016077 @default.
- W2078751476 cites W2063559020 @default.
- W2078751476 cites W2084180910 @default.
- W2078751476 cites W2086732436 @default.
- W2078751476 cites W2108762011 @default.
- W2078751476 doi "https://doi.org/10.1021/ac7017469" @default.
- W2078751476 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17985928" @default.
- W2078751476 hasPublicationYear "2007" @default.
- W2078751476 type Work @default.
- W2078751476 sameAs 2078751476 @default.
- W2078751476 citedByCount "57" @default.
- W2078751476 countsByYear W20787514762012 @default.
- W2078751476 countsByYear W20787514762013 @default.
- W2078751476 countsByYear W20787514762014 @default.
- W2078751476 countsByYear W20787514762015 @default.
- W2078751476 countsByYear W20787514762016 @default.
- W2078751476 countsByYear W20787514762017 @default.
- W2078751476 countsByYear W20787514762018 @default.
- W2078751476 countsByYear W20787514762019 @default.
- W2078751476 countsByYear W20787514762020 @default.
- W2078751476 countsByYear W20787514762021 @default.
- W2078751476 countsByYear W20787514762022 @default.
- W2078751476 countsByYear W20787514762023 @default.
- W2078751476 crossrefType "journal-article" @default.
- W2078751476 hasAuthorship W2078751476A5062806679 @default.
- W2078751476 hasAuthorship W2078751476A5079589390 @default.
- W2078751476 hasAuthorship W2078751476A5091399120 @default.
- W2078751476 hasConcept C108625454 @default.
- W2078751476 hasConcept C147990577 @default.
- W2078751476 hasConcept C162356407 @default.
- W2078751476 hasConcept C170493617 @default.
- W2078751476 hasConcept C179247698 @default.
- W2078751476 hasConcept C181199279 @default.
- W2078751476 hasConcept C185592680 @default.
- W2078751476 hasConcept C206212055 @default.
- W2078751476 hasConcept C2776923573 @default.
- W2078751476 hasConcept C2777313579 @default.
- W2078751476 hasConcept C2779281246 @default.
- W2078751476 hasConcept C31827203 @default.
- W2078751476 hasConcept C43617362 @default.
- W2078751476 hasConcept C55493867 @default.
- W2078751476 hasConceptScore W2078751476C108625454 @default.
- W2078751476 hasConceptScore W2078751476C147990577 @default.
- W2078751476 hasConceptScore W2078751476C162356407 @default.
- W2078751476 hasConceptScore W2078751476C170493617 @default.
- W2078751476 hasConceptScore W2078751476C179247698 @default.
- W2078751476 hasConceptScore W2078751476C181199279 @default.
- W2078751476 hasConceptScore W2078751476C185592680 @default.
- W2078751476 hasConceptScore W2078751476C206212055 @default.
- W2078751476 hasConceptScore W2078751476C2776923573 @default.
- W2078751476 hasConceptScore W2078751476C2777313579 @default.
- W2078751476 hasConceptScore W2078751476C2779281246 @default.
- W2078751476 hasConceptScore W2078751476C31827203 @default.
- W2078751476 hasConceptScore W2078751476C43617362 @default.
- W2078751476 hasConceptScore W2078751476C55493867 @default.
- W2078751476 hasIssue "24" @default.
- W2078751476 hasLocation W20787514761 @default.
- W2078751476 hasLocation W20787514762 @default.
- W2078751476 hasOpenAccess W2078751476 @default.
- W2078751476 hasPrimaryLocation W20787514761 @default.
- W2078751476 hasRelatedWork W1985711808 @default.
- W2078751476 hasRelatedWork W2020742672 @default.
- W2078751476 hasRelatedWork W2055579561 @default.
- W2078751476 hasRelatedWork W2093208477 @default.
- W2078751476 hasRelatedWork W2115218143 @default.
- W2078751476 hasRelatedWork W2467145952 @default.
- W2078751476 hasRelatedWork W2620208828 @default.
- W2078751476 hasRelatedWork W2625646682 @default.
- W2078751476 hasRelatedWork W3153776680 @default.
- W2078751476 hasRelatedWork W3179138806 @default.
- W2078751476 hasVolume "79" @default.
- W2078751476 isParatext "false" @default.
- W2078751476 isRetracted "false" @default.
- W2078751476 magId "2078751476" @default.
- W2078751476 workType "article" @default.