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- W2078753837 abstract "1. Free polysomes and bound polysomes, prepared from rat liver were incubated with [3H]- and [14C]leucine, respectively, in a cell-free system. The labelled supernatant fractions were then combined and fractionated by DEAE-cellulose column chromatography1, followed by acrylamide gel electrophoresis. The serum albumin fraction was found to be preferentially synthesized by bound polysomes. 2. From the supernatant fraction, doubly labelled with [14C]- and [3H]leucine in the cell-free system as described above, the serum albumin fraction and the catalase fraction were prepared by immunological methods i.e. by employing specific antisera against them, after the removal of unspecific proteins. It was found that bound polysomes synthesized serum albumin exclusively. It was further suggested that catalase was synthesized on both kinds of polysomes although the results were not conclusive, owing to the low incorporation of the amino acid into this enzyme fraction. 3. The results of time-course experiments of the incorporation of [14C]leucine in vivo into the serum albumin fraction, which was prepared from the “nascent protein” fractions of both kinds of polysomes by the immunological methods described above, showed that the labelled albumin fraction was located only on bound polysomes and supported the indication of exclusive synthesis of this protein by bound polysomes. 4. The DEAE-cellulose chromatography1 of the supernatant fraction, doubly-labelled by both kinds of polysomes in a cell-free system, showed a fraction (Fraction I) eluted by 15 mM NaH2PO4, which was more effectively synthesized by free polysomes." @default.
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- W2078753837 title "Functional differences in protein synthesis between free and bound polysomes of rat liver" @default.
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- W2078753837 doi "https://doi.org/10.1016/0005-2787(70)90131-0" @default.
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