Matches in SemOpenAlex for { <https://semopenalex.org/work/W2078755596> ?p ?o ?g. }
Showing items 1 to 88 of
88
with 100 items per page.
- W2078755596 endingPage "55" @default.
- W2078755596 startingPage "47" @default.
- W2078755596 abstract "High-resolution confocal laser scanning microscopy (CLSM) is a powerful tool for in situ observation and analysis of protein crystal growth kinetics. Because the resolution of CLSM is not diffraction-limited by the object, it is possible to visualize, under certain conditions, objects in molecular dimensions. A modified batch technique is applied which allows the growth kinetics of sufficiently small crystallites fixed at the lower side of a cover glass, within a hanging drop, to be studied in reflected light near the total reflection angle. A gap, or cavity, filled with solution is formed between the cover glass and the upper crystal face, which acts to fix small crystallites by hydrodynamic friction forces. The cavity height enables the propagation of molecular steps across the upper crystal face without constraint, so that the propagation velocity and geometrical parameters can be measured by CLSM. The layer growth kinetics of monoclinic crystallites of a long-acting insulin derivative (Insulin Glargine) is investigated. For a twofold supersaturation of the solution, the growth is governed by 2D nucleation at the edges of the crystallites followed by a spreading of molecular steps. The layer growth kinetics are well fitted by the simple cubic kinetic lattice model. We find that only about one of a thousand solute (protein) molecules which push a kink place due to their Brownian motion becomes really incorporated into the growing crystal." @default.
- W2078755596 created "2016-06-24" @default.
- W2078755596 creator A5045562414 @default.
- W2078755596 creator A5049892275 @default.
- W2078755596 creator A5058886000 @default.
- W2078755596 creator A5091554343 @default.
- W2078755596 date "2003-04-01" @default.
- W2078755596 modified "2023-09-23" @default.
- W2078755596 title "Noninvasive in situ observation of the crystallization kinetics of biological macromolecules by confocal laser scanning microscopy" @default.
- W2078755596 cites W190932957 @default.
- W2078755596 cites W2006807096 @default.
- W2078755596 cites W2010905503 @default.
- W2078755596 cites W2017964928 @default.
- W2078755596 cites W2018428255 @default.
- W2078755596 cites W2031472272 @default.
- W2078755596 cites W2068775738 @default.
- W2078755596 cites W2078641412 @default.
- W2078755596 cites W2086956760 @default.
- W2078755596 cites W2089707023 @default.
- W2078755596 cites W2951596574 @default.
- W2078755596 doi "https://doi.org/10.1016/s1047-8477(03)00037-6" @default.
- W2078755596 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12718918" @default.
- W2078755596 hasPublicationYear "2003" @default.
- W2078755596 type Work @default.
- W2078755596 sameAs 2078755596 @default.
- W2078755596 citedByCount "7" @default.
- W2078755596 countsByYear W20787555962015 @default.
- W2078755596 crossrefType "journal-article" @default.
- W2078755596 hasAuthorship W2078755596A5045562414 @default.
- W2078755596 hasAuthorship W2078755596A5049892275 @default.
- W2078755596 hasAuthorship W2078755596A5058886000 @default.
- W2078755596 hasAuthorship W2078755596A5091554343 @default.
- W2078755596 hasConcept C120665830 @default.
- W2078755596 hasConcept C121332964 @default.
- W2078755596 hasConcept C137637335 @default.
- W2078755596 hasConcept C147080431 @default.
- W2078755596 hasConcept C148898269 @default.
- W2078755596 hasConcept C159467904 @default.
- W2078755596 hasConcept C178790620 @default.
- W2078755596 hasConcept C185592680 @default.
- W2078755596 hasConcept C192562407 @default.
- W2078755596 hasConcept C199360897 @default.
- W2078755596 hasConcept C203036418 @default.
- W2078755596 hasConcept C26456737 @default.
- W2078755596 hasConcept C2781285689 @default.
- W2078755596 hasConcept C41008148 @default.
- W2078755596 hasConcept C61048295 @default.
- W2078755596 hasConcept C62520636 @default.
- W2078755596 hasConcept C8010536 @default.
- W2078755596 hasConceptScore W2078755596C120665830 @default.
- W2078755596 hasConceptScore W2078755596C121332964 @default.
- W2078755596 hasConceptScore W2078755596C137637335 @default.
- W2078755596 hasConceptScore W2078755596C147080431 @default.
- W2078755596 hasConceptScore W2078755596C148898269 @default.
- W2078755596 hasConceptScore W2078755596C159467904 @default.
- W2078755596 hasConceptScore W2078755596C178790620 @default.
- W2078755596 hasConceptScore W2078755596C185592680 @default.
- W2078755596 hasConceptScore W2078755596C192562407 @default.
- W2078755596 hasConceptScore W2078755596C199360897 @default.
- W2078755596 hasConceptScore W2078755596C203036418 @default.
- W2078755596 hasConceptScore W2078755596C26456737 @default.
- W2078755596 hasConceptScore W2078755596C2781285689 @default.
- W2078755596 hasConceptScore W2078755596C41008148 @default.
- W2078755596 hasConceptScore W2078755596C61048295 @default.
- W2078755596 hasConceptScore W2078755596C62520636 @default.
- W2078755596 hasConceptScore W2078755596C8010536 @default.
- W2078755596 hasIssue "1" @default.
- W2078755596 hasLocation W20787555961 @default.
- W2078755596 hasLocation W20787555962 @default.
- W2078755596 hasOpenAccess W2078755596 @default.
- W2078755596 hasPrimaryLocation W20787555961 @default.
- W2078755596 hasRelatedWork W2039896186 @default.
- W2078755596 hasRelatedWork W2055733920 @default.
- W2078755596 hasRelatedWork W2056078797 @default.
- W2078755596 hasRelatedWork W2072714408 @default.
- W2078755596 hasRelatedWork W2098254896 @default.
- W2078755596 hasRelatedWork W3102222541 @default.
- W2078755596 hasRelatedWork W3103131106 @default.
- W2078755596 hasRelatedWork W3136331550 @default.
- W2078755596 hasRelatedWork W31669409 @default.
- W2078755596 hasRelatedWork W31987158 @default.
- W2078755596 hasVolume "142" @default.
- W2078755596 isParatext "false" @default.
- W2078755596 isRetracted "false" @default.
- W2078755596 magId "2078755596" @default.
- W2078755596 workType "article" @default.