FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2078759462> ?p ?o ?g. }

• W2078759462 endingPage "9768" @default.
• W2078759462 startingPage "9763" @default.
• W2078759462 abstract "Three monoclonal antibodies (Mab) specific for prostate-specific antigen (PSA) were used to design a homogeneous dual-parameter immunoassay based on fluorescence resonance energy transfer (FRET). One antibody was labeled with terbium(III) chelate, which acted as a donor, and the other two antibodies were labeled with fluorescent acceptor dyes (either Alexa Fluor (AF) 488 or Alexa Fluor 680). Due to the selection of the antibodies, sensitized emission of the AF488 could be measured only if uncomplexed, free PSA (PSA-F) was present in the sample. The amount of total PSA (PSA-T) was obtained by measuring the sensitized emission of AF680. Thus, the assay could simultaneously measure the amount of both PSA-F and PSA-T from a single sample. The lowest limits of detection with buffer calibrators were 0.74 and 0.60 ng/mL for PSA-F and PSA-T, respectively. Both of the assays were linear up to 100 ng/mL. The performance of the assay was also tested against heterogeneous single-parameter assays using spiked female plasma samples. The Pearson's correlations were 0.994 for PSA-F and 0.997 for PSA-T assays. However, due to the calibrator matrix being different from the sample matrix, the obtained concentrations with homogeneous assay, especially for PSA-F, were slightly less than with heterogeneous assays. In conclusion, it was shown that a homogeneous dual-parameter assay based on the measurement of the sensitized emission of two different acceptors in combination with a single donor can be performed. The assay was done in a single step using only one excitation wavelength and was functional within the clinically important analyte concentrations." @default.
• W2078759462 created "2016-06-24" @default.
• W2078759462 creator A5006024422 @default.
• W2078759462 creator A5040921944 @default.
• W2078759462 creator A5049015452 @default.
• W2078759462 creator A5068834945 @default.
• W2078759462 creator A5079414719 @default.
• W2078759462 date "2008-11-14" @default.
• W2078759462 modified "2023-09-27" @default.
• W2078759462 title "Homogeneous Dual-Parameter Assay for Prostate-Specific Antigen Based on Fluorescence Resonance Energy Transfer" @default.
• W2078759462 cites W1617221784 @default.
• W2078759462 cites W1759125113 @default.
• W2078759462 cites W1845134010 @default.
• W2078759462 cites W1847856759 @default.
• W2078759462 cites W1989294263 @default.
• W2078759462 cites W2014017554 @default.
• W2078759462 cites W2025583455 @default.
• W2078759462 cites W2036856449 @default.
• W2078759462 cites W2037513141 @default.
• W2078759462 cites W2041054894 @default.
• W2078759462 cites W2059546764 @default.
• W2078759462 cites W2074158320 @default.
• W2078759462 cites W2083062424 @default.
• W2078759462 cites W2085756391 @default.
• W2078759462 cites W2094517427 @default.
• W2078759462 cites W2095083074 @default.
• W2078759462 cites W2098394831 @default.
• W2078759462 cites W2119408931 @default.
• W2078759462 cites W2119823606 @default.
• W2078759462 cites W2120517440 @default.
• W2078759462 cites W2133756745 @default.
• W2078759462 cites W2138678394 @default.
• W2078759462 cites W2155645504 @default.
• W2078759462 cites W2165507507 @default.
• W2078759462 cites W2170901363 @default.
• W2078759462 cites W2321493387 @default.
• W2078759462 cites W4295063935 @default.
• W2078759462 cites W4360754451 @default.
• W2078759462 doi "https://doi.org/10.1021/ac801875a" @default.
• W2078759462 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19012418" @default.
• W2078759462 hasPublicationYear "2008" @default.
• W2078759462 type Work @default.
• W2078759462 sameAs 2078759462 @default.
• W2078759462 citedByCount "43" @default.
• W2078759462 countsByYear W20787594622012 @default.
• W2078759462 countsByYear W20787594622013 @default.
• W2078759462 countsByYear W20787594622014 @default.
• W2078759462 countsByYear W20787594622015 @default.
• W2078759462 countsByYear W20787594622016 @default.
• W2078759462 countsByYear W20787594622017 @default.
• W2078759462 countsByYear W20787594622018 @default.
• W2078759462 countsByYear W20787594622019 @default.
• W2078759462 countsByYear W20787594622020 @default.
• W2078759462 countsByYear W20787594622021 @default.
• W2078759462 countsByYear W20787594622022 @default.
• W2078759462 crossrefType "journal-article" @default.
• W2078759462 hasAuthorship W2078759462A5006024422 @default.
• W2078759462 hasAuthorship W2078759462A5040921944 @default.
• W2078759462 hasAuthorship W2078759462A5049015452 @default.
• W2078759462 hasAuthorship W2078759462A5068834945 @default.
• W2078759462 hasAuthorship W2078759462A5079414719 @default.
• W2078759462 hasConcept C113196181 @default.
• W2078759462 hasConcept C119128265 @default.
• W2078759462 hasConcept C120665830 @default.
• W2078759462 hasConcept C121332964 @default.
• W2078759462 hasConcept C121608353 @default.
• W2078759462 hasConcept C126322002 @default.
• W2078759462 hasConcept C147483822 @default.
• W2078759462 hasConcept C153911025 @default.
• W2078759462 hasConcept C159654299 @default.
• W2078759462 hasConcept C185592680 @default.
• W2078759462 hasConcept C203014093 @default.
• W2078759462 hasConcept C2776235491 @default.
• W2078759462 hasConcept C2776759294 @default.
• W2078759462 hasConcept C2778015030 @default.
• W2078759462 hasConcept C2780420688 @default.
• W2078759462 hasConcept C2781406297 @default.
• W2078759462 hasConcept C43617362 @default.
• W2078759462 hasConcept C66882249 @default.
• W2078759462 hasConcept C71924100 @default.
• W2078759462 hasConcept C86803240 @default.
• W2078759462 hasConcept C91881484 @default.
• W2078759462 hasConcept C96305047 @default.
• W2078759462 hasConcept C97355855 @default.
• W2078759462 hasConceptScore W2078759462C113196181 @default.
• W2078759462 hasConceptScore W2078759462C119128265 @default.
• W2078759462 hasConceptScore W2078759462C120665830 @default.
• W2078759462 hasConceptScore W2078759462C121332964 @default.
• W2078759462 hasConceptScore W2078759462C121608353 @default.
• W2078759462 hasConceptScore W2078759462C126322002 @default.
• W2078759462 hasConceptScore W2078759462C147483822 @default.
• W2078759462 hasConceptScore W2078759462C153911025 @default.
• W2078759462 hasConceptScore W2078759462C159654299 @default.
• W2078759462 hasConceptScore W2078759462C185592680 @default.
• W2078759462 hasConceptScore W2078759462C203014093 @default.
• W2078759462 hasConceptScore W2078759462C2776235491 @default.
• W2078759462 hasConceptScore W2078759462C2776759294 @default.
• W2078759462 hasConceptScore W2078759462C2778015030 @default.

• next