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- W2078773713 abstract "High-dose methotrexate therapy with following citrovorum factor rescue necessitates the regular determinations of methotrexate (MTX) serum concentrations. A simple enzymatic assay by MTX induced inhibition of dihydrofolate reductase is described. The results gained with this assay are compared with those of a new enzyme immunoassay (MTX-EMIT). Between 5 X 10(-7) M and 1 X 10(-3) M the results of both tests are nearly the same, and the coefficient of correlation is 0,9980. At MTX concentrations below 5 X 10(-7) M the results of the MTX-EMIT lie nearly always higher than and differ considerably from those of the enzymatic method; the coefficient of correlation is only 0.7007. If it is possible to enhance the sensitivity of the MTX-EMIT, this test enables the very quick and easy determination of MTX serum concentrations in the whole range of clinical importance." @default.
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- W2078773713 date "1980-01-01" @default.
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- W2078773713 title "Methotrexat-Nachweis im menschlichen Serum – Vergleich zwischen einer enzymatischen und einer enzymimmunologischen Methode" @default.
- W2078773713 doi "https://doi.org/10.1159/000214791" @default.
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