Matches in SemOpenAlex for { <https://semopenalex.org/work/W2078774598> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W2078774598 endingPage "58" @default.
- W2078774598 startingPage "53" @default.
- W2078774598 abstract "Newly prepared phosphatidylethanolamine (PE) conjugates of glycosaminoglycans can be immobilized to solid phase through hydrophobic interaction. Primary rat hepatocytes were cultured on type I collagen or laminin substrates containing heparin-PE and assayed for DNA synthesis initiated by hepatocyte growth factor (HGF). Hepatocytes responded to HGF that had been added to culture media by active DNA synthesis and pronounced cell spreading, regardless of the presence of heparin-PE on the substrates. The preincubation with HGF, but not with epidermal growth factor, of the substrates containing heparin-PE significantly enhanced DNA synthesis and cell spreading in the absence of these mitogens in culture media. The enhancements were abolished by omitting heparin-PE or washing the HGF-treated substrates with 1 M NaCl, suggesting the immobilization of HGF to substrates through heparin. These phenomena were more evident with laminin substrates than with type I collagen substrates. Chondroitin sulfate-PE only partly substituted for heparin-PE, but Matrigel gave results similar to those of the substrates containing heparin-PE. Both type I collagen and laminin substrates containing heparin-PE bound higher amounts of HGF than the respective control substrates, whereas neither of the substrates containing chondroitin sulfate-PE did. However, the increase in HGF binding to the substrates was not as evident as the increase in DNA synthesis, suggesting that the latter is not simply due to the former but due to a concerted action of the immobilized HGF and heparin. Taken together, these results suggest that HGF can be trapped in extracellular matrix, probably through heparan sulfate in vivo, thereby acting as a mitogen for hepatocytes in cooperation with heparan sulfate." @default.
- W2078774598 created "2016-06-24" @default.
- W2078774598 creator A5015487871 @default.
- W2078774598 creator A5027855293 @default.
- W2078774598 creator A5048896544 @default.
- W2078774598 creator A5056589245 @default.
- W2078774598 creator A5067793902 @default.
- W2078774598 creator A5085195548 @default.
- W2078774598 date "1994-03-01" @default.
- W2078774598 modified "2023-09-23" @default.
- W2078774598 title "Hepatocyte Growth Factor Immobilized onto Culture Substrates through Heparin and Matrigel Enhances DNA Synthesis in Primary Rat Hepatocytes" @default.
- W2078774598 doi "https://doi.org/10.1006/excr.1994.1058" @default.
- W2078774598 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8125158" @default.
- W2078774598 hasPublicationYear "1994" @default.
- W2078774598 type Work @default.
- W2078774598 sameAs 2078774598 @default.
- W2078774598 citedByCount "34" @default.
- W2078774598 countsByYear W20787745982012 @default.
- W2078774598 countsByYear W20787745982013 @default.
- W2078774598 countsByYear W20787745982015 @default.
- W2078774598 countsByYear W20787745982016 @default.
- W2078774598 countsByYear W20787745982018 @default.
- W2078774598 countsByYear W20787745982019 @default.
- W2078774598 countsByYear W20787745982022 @default.
- W2078774598 crossrefType "journal-article" @default.
- W2078774598 hasAuthorship W2078774598A5015487871 @default.
- W2078774598 hasAuthorship W2078774598A5027855293 @default.
- W2078774598 hasAuthorship W2078774598A5048896544 @default.
- W2078774598 hasAuthorship W2078774598A5056589245 @default.
- W2078774598 hasAuthorship W2078774598A5067793902 @default.
- W2078774598 hasAuthorship W2078774598A5085195548 @default.
- W2078774598 hasConcept C1491633281 @default.
- W2078774598 hasConcept C153074725 @default.
- W2078774598 hasConcept C153911025 @default.
- W2078774598 hasConcept C170493617 @default.
- W2078774598 hasConcept C189165786 @default.
- W2078774598 hasConcept C2775960820 @default.
- W2078774598 hasConcept C2776996007 @default.
- W2078774598 hasConcept C2777107211 @default.
- W2078774598 hasConcept C2777557582 @default.
- W2078774598 hasConcept C2778041096 @default.
- W2078774598 hasConcept C2778608917 @default.
- W2078774598 hasConcept C2779553658 @default.
- W2078774598 hasConcept C2779814568 @default.
- W2078774598 hasConcept C54355233 @default.
- W2078774598 hasConcept C552990157 @default.
- W2078774598 hasConcept C55493867 @default.
- W2078774598 hasConcept C64241487 @default.
- W2078774598 hasConcept C81885089 @default.
- W2078774598 hasConcept C86803240 @default.
- W2078774598 hasConceptScore W2078774598C1491633281 @default.
- W2078774598 hasConceptScore W2078774598C153074725 @default.
- W2078774598 hasConceptScore W2078774598C153911025 @default.
- W2078774598 hasConceptScore W2078774598C170493617 @default.
- W2078774598 hasConceptScore W2078774598C189165786 @default.
- W2078774598 hasConceptScore W2078774598C2775960820 @default.
- W2078774598 hasConceptScore W2078774598C2776996007 @default.
- W2078774598 hasConceptScore W2078774598C2777107211 @default.
- W2078774598 hasConceptScore W2078774598C2777557582 @default.
- W2078774598 hasConceptScore W2078774598C2778041096 @default.
- W2078774598 hasConceptScore W2078774598C2778608917 @default.
- W2078774598 hasConceptScore W2078774598C2779553658 @default.
- W2078774598 hasConceptScore W2078774598C2779814568 @default.
- W2078774598 hasConceptScore W2078774598C54355233 @default.
- W2078774598 hasConceptScore W2078774598C552990157 @default.
- W2078774598 hasConceptScore W2078774598C55493867 @default.
- W2078774598 hasConceptScore W2078774598C64241487 @default.
- W2078774598 hasConceptScore W2078774598C81885089 @default.
- W2078774598 hasConceptScore W2078774598C86803240 @default.
- W2078774598 hasIssue "1" @default.
- W2078774598 hasLocation W20787745981 @default.
- W2078774598 hasLocation W20787745982 @default.
- W2078774598 hasOpenAccess W2078774598 @default.
- W2078774598 hasPrimaryLocation W20787745981 @default.
- W2078774598 hasRelatedWork W1584933943 @default.
- W2078774598 hasRelatedWork W1994734003 @default.
- W2078774598 hasRelatedWork W2048706959 @default.
- W2078774598 hasRelatedWork W2068473054 @default.
- W2078774598 hasRelatedWork W2077975783 @default.
- W2078774598 hasRelatedWork W2078774598 @default.
- W2078774598 hasRelatedWork W2161132730 @default.
- W2078774598 hasRelatedWork W2402077000 @default.
- W2078774598 hasRelatedWork W2973215277 @default.
- W2078774598 hasRelatedWork W4244891144 @default.
- W2078774598 hasVolume "211" @default.
- W2078774598 isParatext "false" @default.
- W2078774598 isRetracted "false" @default.
- W2078774598 magId "2078774598" @default.
- W2078774598 workType "article" @default.