FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2078778234> ?p ?o ?g. }

• W2078778234 endingPage "2896" @default.
• W2078778234 startingPage "2889" @default.
• W2078778234 abstract "Separate demonstration of immunoreactive (ir) atrial natriuretic factor (ANF) and ir-beta-endorphin (beta EP) in macrophages of the rat spleen prompted a reexamination of their distribution and cellular localization in addition to their developmental regulation. Double labeling immunohistochemistry was carried out on paraffin-embedded spleen sections of adult male Sprague-Dawley rats. Cells stained positive with antiserum (S118) raised against rat ANF-(99-126) were colocalized in more than 95% of cases with immunofluorescent staining of ir beta EP-(1-31) and distributed sparsely throughout the venous sinusoidal regions of the red pulp. In 1- or 5-day monolayer cultures of adherent splenocytes, 11-16% of the cells stained positive for either irANF or ir beta EP. Under these conditions, more than 95% of irANF- and ir beta EP-positive cells were also fluorescence stained for the histiocyte marker S22 or the rat macrophage marker ED-1. Colorimetric in situ hybridization similarly revealed signals for pro-ANF mRNA in more than 95% of ir beta EP-positive adherent cells. Thus, taken together with previous reports, our present findings suggest that in the rat spleen, both ANF and beta EP are produced by the same population of macrophages. However, the tissue levels and processing of the two peptides over the developmental period of the animal differed markedly in several ways. In splenic extracts of 2-day-old neonatal rats, Northern blot analysis and RIA revealed a greater abundance of pro-ANF mRNA signal and an irANF concentration about 6-fold greater than those in 30-day-old animals. In contrast, the ir beta EP concentration did not vary significantly over the same period, consistent with the level of POMC mRNA. Sephadex G-50 gel chromatography of splenic extracts from 2-day-old animals revealed predominantly higher mol wt forms of irANF and ir beta EP. From days 16-60, a significant proportion of irANF eluted in the same fractions as the mature circulating form, rat ANF-(99-126); the proportion of 3.5-kilodalton ir beta EP increased progressively to become the predominant species in adult tissues. Thus, it appears that although ANF and beta EP are coexpressed by the same population of splenic macrophages, they differ markedly during the developmental period with respect to their constitutive regulation." @default.
• W2078778234 created "2016-06-24" @default.
• W2078778234 creator A5015257126 @default.
• W2078778234 creator A5028504214 @default.
• W2078778234 creator A5076108959 @default.
• W2078778234 creator A5078833852 @default.
• W2078778234 creator A5079896090 @default.
• W2078778234 creator A5085393932 @default.
• W2078778234 date "1993-12-01" @default.
• W2078778234 modified "2023-09-27" @default.
• W2078778234 title "Coexpression of atrial natriuretic factor and beta-endorphin in a subpopulation of rat splenic macrophages: age-related differences." @default.
• W2078778234 cites W10533360 @default.
• W2078778234 cites W1509771501 @default.
• W2078778234 cites W1617037549 @default.
• W2078778234 cites W1967295182 @default.
• W2078778234 cites W1969809783 @default.
• W2078778234 cites W1973773253 @default.
• W2078778234 cites W1996558440 @default.
• W2078778234 cites W2025289359 @default.
• W2078778234 cites W2040714787 @default.
• W2078778234 cites W2054844061 @default.
• W2078778234 cites W2089252496 @default.
• W2078778234 cites W2095736624 @default.
• W2078778234 cites W2098345014 @default.
• W2078778234 cites W2098568602 @default.
• W2078778234 cites W2120173848 @default.
• W2078778234 cites W2148887671 @default.
• W2078778234 cites W2161497477 @default.
• W2078778234 cites W1498231868 @default.
• W2078778234 doi "https://doi.org/10.1210/endo.133.6.8243316" @default.
• W2078778234 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8243316" @default.
• W2078778234 hasPublicationYear "1993" @default.
• W2078778234 type Work @default.
• W2078778234 sameAs 2078778234 @default.
• W2078778234 citedByCount "6" @default.
• W2078778234 crossrefType "journal-article" @default.
• W2078778234 hasAuthorship W2078778234A5015257126 @default.
• W2078778234 hasAuthorship W2078778234A5028504214 @default.
• W2078778234 hasAuthorship W2078778234A5076108959 @default.
• W2078778234 hasAuthorship W2078778234A5078833852 @default.
• W2078778234 hasAuthorship W2078778234A5079896090 @default.
• W2078778234 hasAuthorship W2078778234A5085393932 @default.
• W2078778234 hasConcept C104317684 @default.
• W2078778234 hasConcept C105580179 @default.
• W2078778234 hasConcept C126322002 @default.
• W2078778234 hasConcept C134018914 @default.
• W2078778234 hasConcept C153911025 @default.
• W2078778234 hasConcept C189976730 @default.
• W2078778234 hasConcept C199360897 @default.
• W2078778234 hasConcept C203014093 @default.
• W2078778234 hasConcept C204232928 @default.
• W2078778234 hasConcept C2776174256 @default.
• W2078778234 hasConcept C2776415932 @default.
• W2078778234 hasConcept C2777092883 @default.
• W2078778234 hasConcept C2780931953 @default.
• W2078778234 hasConcept C2908647359 @default.
• W2078778234 hasConcept C35866371 @default.
• W2078778234 hasConcept C41008148 @default.
• W2078778234 hasConcept C54355233 @default.
• W2078778234 hasConcept C55493867 @default.
• W2078778234 hasConcept C71924100 @default.
• W2078778234 hasConcept C74864618 @default.
• W2078778234 hasConcept C81439078 @default.
• W2078778234 hasConcept C86803240 @default.
• W2078778234 hasConcept C99454951 @default.
• W2078778234 hasConceptScore W2078778234C104317684 @default.
• W2078778234 hasConceptScore W2078778234C105580179 @default.
• W2078778234 hasConceptScore W2078778234C126322002 @default.
• W2078778234 hasConceptScore W2078778234C134018914 @default.
• W2078778234 hasConceptScore W2078778234C153911025 @default.
• W2078778234 hasConceptScore W2078778234C189976730 @default.
• W2078778234 hasConceptScore W2078778234C199360897 @default.
• W2078778234 hasConceptScore W2078778234C203014093 @default.
• W2078778234 hasConceptScore W2078778234C204232928 @default.
• W2078778234 hasConceptScore W2078778234C2776174256 @default.
• W2078778234 hasConceptScore W2078778234C2776415932 @default.
• W2078778234 hasConceptScore W2078778234C2777092883 @default.
• W2078778234 hasConceptScore W2078778234C2780931953 @default.
• W2078778234 hasConceptScore W2078778234C2908647359 @default.
• W2078778234 hasConceptScore W2078778234C35866371 @default.
• W2078778234 hasConceptScore W2078778234C41008148 @default.
• W2078778234 hasConceptScore W2078778234C54355233 @default.
• W2078778234 hasConceptScore W2078778234C55493867 @default.
• W2078778234 hasConceptScore W2078778234C71924100 @default.
• W2078778234 hasConceptScore W2078778234C74864618 @default.
• W2078778234 hasConceptScore W2078778234C81439078 @default.
• W2078778234 hasConceptScore W2078778234C86803240 @default.
• W2078778234 hasConceptScore W2078778234C99454951 @default.
• W2078778234 hasIssue "6" @default.
• W2078778234 hasLocation W20787782341 @default.
• W2078778234 hasLocation W20787782342 @default.
• W2078778234 hasOpenAccess W2078778234 @default.
• W2078778234 hasPrimaryLocation W20787782341 @default.
• W2078778234 hasRelatedWork W1978332820 @default.
• W2078778234 hasRelatedWork W2044918899 @default.
• W2078778234 hasRelatedWork W2047539822 @default.
• W2078778234 hasRelatedWork W2054143056 @default.
• W2078778234 hasRelatedWork W2078778234 @default.

• next