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- W2078785159 abstract "The nucleotide sequence of a cDNA clone for barley glyceraldehyde-3-phosphate dehydrogenase (GAPDH) has been determined. The cDNA is 1147 base pairs long, encoding 305 amino-acids of the GAPDH polypeptide. Comparison of the amino acid sequence, predicted from the DNA sequence, with GAPDH sequences available for other organisms reveals a high degree of sequence conservation, particularly in regions of supposed importance for tertiary structure and activity. Hybridisation of the cDNA insert to barley genomic DNA indicates that there is a single copy of the gene per haploid genome. The expression of the GAPDH gene in the scutellum during germination, as detected by RNA hydridisation analysis, probably reflects the involvement of the enzyme at key steps in both the glycolytic and gluconeogenic pathways." @default.
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- W2078785159 date "1986-01-01" @default.
- W2078785159 modified "2023-09-26" @default.
- W2078785159 title "Identification and characterisation of a cDNA clone for cytosolic glyceraldehyde-3-phosphate dehydrogenase in barley" @default.
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- W2078785159 doi "https://doi.org/10.1007/bf02907324" @default.
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