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• W2078792038 abstract "An mRNA differential display methodology was used to study the rainbow trout response to viral infection. A new transcript (vig -2) induced by viral haemorrhagic septicaemia virus (VHSV) in rainbow trout leucocytes was identified from the head-kidney. vig -2 was also induced in vivo during experimental infection and following DNA immunisation with a plasmid containing a gene encoding the viral glycoprotein. Viral induction of vig -2 was blocked by cycloheximide (CHX), indicating its dependency on a newly synthesised intermediate protein. This intermediate protein is most probably related to interferon because treatment of cells with a conditioned medium displaying an interferon-like activity resulted in a strong vig -2 expression, which was not blocked by CHX treatment. The cDNA sequence of the vig -2 transcript displays several mRNA destabilisation motifs and two signals characteristic of immediate–early gene expression. Curiously,vig -2 has no evident encoding potential except for a small 51 amino acid putative polypeptide with no clear similarity to any sequence available in the databanks. Therefore, the complete vig -2 genomic sequence was determined from a lambda phage clone retrieved from a genomic DNA library of rainbow trout. The genomic organisation of vig -2 shows five exons delimited with typical splice acceptor and donor sites. A promoter with a canonical ISRE, confirming that vig -2 is an interferon-responsive gene, is also present 115 nt upstream of the first exon." @default.
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• W2078792038 date "2001-07-01" @default.
• W2078792038 modified "2023-10-09" @default.
• W2078792038 title "Viral haemorrhagic septicaemia virus induces vig -2, a new interferon-responsive gene in rainbow trout" @default.
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• W2078792038 doi "https://doi.org/10.1006/fsim.2000.0326" @default.
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