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• W2078792659 abstract "RNA interference methodology suppresses gene expression, thus mimicking loss-of-function mutation and enabling in vitro and in vivo gene function analysis. In this study, we used retroviral and lentiviral vectors to deliver small interfering RNAs and report high-efficiency silencing of a green fluorescent protein (GFP) trans gene and the stem cell-specific transcription factors Oct4/POU5F1 and Nanog in human embryonic stem cells. Gene knockdown of Oct4 and Nanog promotes differentiation, thereby demonstrating a role for these factors in human embryonic stem cell self-renewal." @default.
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• W2078792659 date "2005-03-01" @default.
• W2078792659 modified "2023-10-11" @default.
• W2078792659 title "High‐Efficiency RNA Interference in Human Embryonic Stem Cells" @default.
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• W2078792659 doi "https://doi.org/10.1634/stemcells.2004-0252" @default.
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