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• W2078839150 abstract "<p>The aim of the present work was the development of a novel amperometric biosensor for rapid detection of sucrose in fruit juices samples. Two enzymes, invertase and fructose dehydrogenase (FDH), were immobilized onto a single-walled carbon nanotube paste (SWCNTP) electrode by wiring with a highly original osmium-polymer hydrogel. A second biosensor, for fructose only, was constructed containing inactive invertase and used for signal subtraction.</p> <p>The biosensor exhibits a detection limit for sucrose of 2 mM, linearity up to 5 mM, good sensitivity of 1.98 mA cm^-2 mM, good reproducibility (RSD = 2.5%), fast response time (8s) and a stability of 4 months if kept under wet conditions at 4 °C.</p> <p>The biosensor was successively tested for specific detection of sucrose and fructose in several commercial fruit juice samples and the results were compared with those obtained with a commercial spectrophotometric enzymatic kit.</p>" @default.
• W2078839150 created "2016-06-24" @default.
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• W2078839150 date "2014-05-04" @default.
• W2078839150 modified "2023-09-26" @default.
• W2078839150 title "Rapid Determination of Sucrose in Fruit Juices: A New Sensitive Carbon Nanotube Paste Osmium-Polymer Mediated Biosensor" @default.
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• W2078839150 doi "https://doi.org/10.5539/jfr.v3n4p101" @default.
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