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- W2078840286 abstract "Abstract A sensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) for anti-thyroglobulin IgG in serum is described. Anti-thyroglobulin IgG in serum was reacted simultaneously with 2,4-dinitrophenyl-thyroglobulin and biotinyl-thyroglobulin. The immune complex formed of the three components was trapped onto polystyrene balls coated with (anti-2,4-dinitrophenyl group) IgG and, after washing, reacted with streptavidin-β-D-galactosidase conjugate. After washing, the immune complex was eluted from the polystyrene balls with εN-2,4-dinitrophenyl-L-lysine and transferred to polystyrene balls coated with (anti-human IgG γ-chain) IgG. β-D-Galactosidase activity bound to the last polystyrene balls was assayed by fluorometry. Biotinylthyroglobulin and streptavidin-β-D-galactosidase conjugate could be prepared more easily than thyroglobulin-β-D-galactosidase conjugate used in the previous immunoassay. Inactive β-D-galactosidase, used to eliminate interference by anti-β-D-galactosidase antibodies in the previous immunoassay, was not required. The present immunoassay was 300-fold more sensitive than the conventional enzyme immunoassay, although 10-fold less sensitive than the previous immunoassay. Antithyroglobulin IgG was demonstrated in all patients with autoimmune thyroid diseases and 54% of healthy subjects." @default.
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- W2078840286 date "1993-10-01" @default.
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- W2078840286 title "Immune Complex Transfer Enzyme Immunoassay for Anti-Thyroglobulin IgG Using 2,4-Dinitrophenyl-thyroglobulin, Biotinyl-thyroglobulin and Streptavidin-β-D-galactosidase Conjugate" @default.
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- W2078840286 doi "https://doi.org/10.1080/00032719308017458" @default.
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