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• W2078844404 abstract "Diesel exhaust and wood burning are important sources of ambient atmospheric particles due to increasing numbers of diesel cars and the importance of wood as a source of renewable energy. Inhalation is the predominant route of entry and uptake for fine and ultrafine particles into the body. Health effects of atmospheric particles are still not completely understood. There is consistent evidence from epidemiology that particle exposure contributes to respiratory and cardiovascular diseases. This study aimed at examining acute responses of airway epithelial cells and luminal macrophages after exposure to freshly emitted and photochemically aged carbonaceous aerosols under realistic atmospheric conditions. In addition to a bronchial epithelial cell line advanced cell cultures namely fully differentiated respiratory epithelia and primary surface macrophages were used. Our results demonstrate that a single exposure of the cells to realistic particle doses of 0.3–3 ng diesel or 3–9 ng wood aerosol per cm 2 cell surface induces small, particle-specific responses. The release of interleukin-6 and -8 was found to be decreased in differentiated airway epithelia but not in the other cell models studied. Aerosol exposure decreased macrophage phagocytic activity by 45–90%. Cell and tissue integrity remained unaffected. Overall, primary and aged particles from the same combustion induced similar responses in the cell models tested, whereby particles from diesel exhaust affected the cells more than those from wood combustion. ► Study on effects of wood and diesel combustion particles on lung cells in-vitro. ► Realistic exposure of cells to freshly emitted and atmospherically aged particles. ► Particle exposure induced subtle changes in cellular functions. ► Primary and aged particles from the same combustion induced similar effects." @default.
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• W2078844404 date "2013-04-01" @default.
• W2078844404 modified "2023-10-17" @default.
• W2078844404 title "Responses of lung cells to realistic exposure of primary and aged carbonaceous aerosols" @default.
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• W2078844404 doi "https://doi.org/10.1016/j.atmosenv.2012.11.055" @default.
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