FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2078890177> ?p ?o ?g. }

• W2078890177 endingPage "2110" @default.
• W2078890177 startingPage "2103" @default.
• W2078890177 abstract "Perturbation of iron metabolism, especially the increase of serum ferritin levels, is often associated with both inflammation and hypertension. Changes in iron availability can affect an important regulator of vascular tone, the endothelial nitric oxide synthase (eNOS), activated by a heme-dependent dimerization.To study the regulation of the anti-hypertensive eNOS in human endothelial cells, in correlation with iron metabolism alterations and stimuli triggering them in vivo, such as inflammation or infection.Cells were treated with stimuli mimicking infection or inflammation [lipopolysaccharide (LPS) and/or tumor necrosis factor alpha (TNFalpha)]. and iron shortage (succinylacetone and desferrioxamine). The effect on eNOS expression and activation was evaluated, as well as ferritin content.eNOS protein expression was evaluated by separating the monomeric from the active dimeric form by low-temperature sodium dodecyl sulphate poly-acrylamide gel electrophoresis (SDS-PAGE), and mRNA was analyzed by semi-quantitative reverse transcriptase (RT)-polymerase chain reaction (PCR). As for LPS and TNFalpha, eNOS monomer decreased already after a 72-h treatment and further at 144 h, whereas the down-regulation of the dimer was slower, peaking at 144 h. Succinylacetone and desferrioxamine were effective only at 144 h. The mRNA levels were increasingly reduced after incubation, more markedly by LPS and TNFalpha together, whereas succinylacetone and desferrioxamine had no effect on transcription. We found that endothelial cells are not the source of increased ferritin production.The results of this study suggest a down-regulating effect of infectious and inflammatory stimuli on eNOS expression, both at the mRNA level and protein expression or stability and dimerization, enhanced by heme and iron shortage, and indicate eNOS as a possible link between infection and hypertension." @default.
• W2078890177 created "2016-06-24" @default.
• W2078890177 creator A5031315170 @default.
• W2078890177 creator A5042199091 @default.
• W2078890177 creator A5062956031 @default.
• W2078890177 creator A5084048577 @default.
• W2078890177 creator A5084455839 @default.
• W2078890177 date "2003-11-01" @default.
• W2078890177 modified "2023-10-14" @default.
• W2078890177 title "Infectious and inflammatory stimuli decrease endothelial nitric oxide synthase activity in vitro" @default.
• W2078890177 cites W1554698754 @default.
• W2078890177 cites W1851149345 @default.
• W2078890177 cites W1966218780 @default.
• W2078890177 cites W1968983777 @default.
• W2078890177 cites W1980034398 @default.
• W2078890177 cites W1987267250 @default.
• W2078890177 cites W1990367048 @default.
• W2078890177 cites W2017673011 @default.
• W2078890177 cites W2039421276 @default.
• W2078890177 cites W2041052090 @default.
• W2078890177 cites W2043650286 @default.
• W2078890177 cites W2049858284 @default.
• W2078890177 cites W2049993098 @default.
• W2078890177 cites W2055037877 @default.
• W2078890177 cites W2055099663 @default.
• W2078890177 cites W2056780690 @default.
• W2078890177 cites W2058785850 @default.
• W2078890177 cites W2061569690 @default.
• W2078890177 cites W2065182000 @default.
• W2078890177 cites W2066383233 @default.
• W2078890177 cites W2074533466 @default.
• W2078890177 cites W2076639542 @default.
• W2078890177 cites W2091499945 @default.
• W2078890177 cites W2094881557 @default.
• W2078890177 cites W2096043946 @default.
• W2078890177 cites W2101866533 @default.
• W2078890177 cites W2101907617 @default.
• W2078890177 cites W2102595278 @default.
• W2078890177 cites W2104438864 @default.
• W2078890177 cites W2120929548 @default.
• W2078890177 cites W2125276450 @default.
• W2078890177 cites W2134382614 @default.
• W2078890177 cites W2145502354 @default.
• W2078890177 cites W2145565857 @default.
• W2078890177 cites W2145570729 @default.
• W2078890177 cites W2149250436 @default.
• W2078890177 cites W2149617435 @default.
• W2078890177 cites W2164608636 @default.
• W2078890177 cites W2258966438 @default.
• W2078890177 cites W2337405092 @default.
• W2078890177 cites W2343162401 @default.
• W2078890177 doi "https://doi.org/10.1097/00004872-200311000-00020" @default.
• W2078890177 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14597854" @default.
• W2078890177 hasPublicationYear "2003" @default.
• W2078890177 type Work @default.
• W2078890177 sameAs 2078890177 @default.
• W2078890177 citedByCount "43" @default.
• W2078890177 countsByYear W20788901772012 @default.
• W2078890177 countsByYear W20788901772013 @default.
• W2078890177 countsByYear W20788901772014 @default.
• W2078890177 countsByYear W20788901772015 @default.
• W2078890177 countsByYear W20788901772017 @default.
• W2078890177 countsByYear W20788901772018 @default.
• W2078890177 countsByYear W20788901772019 @default.
• W2078890177 countsByYear W20788901772020 @default.
• W2078890177 countsByYear W20788901772021 @default.
• W2078890177 crossrefType "journal-article" @default.
• W2078890177 hasAuthorship W2078890177A5031315170 @default.
• W2078890177 hasAuthorship W2078890177A5042199091 @default.
• W2078890177 hasAuthorship W2078890177A5062956031 @default.
• W2078890177 hasAuthorship W2078890177A5084048577 @default.
• W2078890177 hasAuthorship W2078890177A5084455839 @default.
• W2078890177 hasConcept C126322002 @default.
• W2078890177 hasConcept C134018914 @default.
• W2078890177 hasConcept C17991360 @default.
• W2078890177 hasConcept C185592680 @default.
• W2078890177 hasConcept C2776914184 @default.
• W2078890177 hasConcept C2777622882 @default.
• W2078890177 hasConcept C2778319317 @default.
• W2078890177 hasConcept C2778326061 @default.
• W2078890177 hasConcept C2778754761 @default.
• W2078890177 hasConcept C2908688039 @default.
• W2078890177 hasConcept C519581460 @default.
• W2078890177 hasConcept C55493867 @default.
• W2078890177 hasConcept C71924100 @default.
• W2078890177 hasConceptScore W2078890177C126322002 @default.
• W2078890177 hasConceptScore W2078890177C134018914 @default.
• W2078890177 hasConceptScore W2078890177C17991360 @default.
• W2078890177 hasConceptScore W2078890177C185592680 @default.
• W2078890177 hasConceptScore W2078890177C2776914184 @default.
• W2078890177 hasConceptScore W2078890177C2777622882 @default.
• W2078890177 hasConceptScore W2078890177C2778319317 @default.
• W2078890177 hasConceptScore W2078890177C2778326061 @default.
• W2078890177 hasConceptScore W2078890177C2778754761 @default.
• W2078890177 hasConceptScore W2078890177C2908688039 @default.
• W2078890177 hasConceptScore W2078890177C519581460 @default.
• W2078890177 hasConceptScore W2078890177C55493867 @default.
• W2078890177 hasConceptScore W2078890177C71924100 @default.

• next