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- W2078903436 abstract "The application of an automated on-line multi-dimensional liquid-liquid chromatrographic technique for the clean-up and analysis of water-soluble samples was investigated. The use of microparticulate aqueous-compatible steric exclusion columns as the primary separation step coupled to either reversed-phase, normal-phase or ion-exchange columns as the secondary step allowed the direct injection of complex samples without prior clean-up. The entire operation was automatically controlled by a microprocessor-based liquid chromatograph with time-programmable events which allowed precise switching of high-pressure pneumatically operated valves. Both heart-cutting and on-column concentration methods were used. The heart-cutting technique had the advantage of selectivity but lacked sensitivity; more successful was the on-column concentration technique, which, by the concentration of the solute from a larger volume of exclusion column effluent on to the secondary column, gave better sensitivity. The technique was applied to the analysis of theophylline and caffeine in biological fluids, catecholamines in urine, vitamins inb a protein food supplement and sugars in molasses and candy bars." @default.
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- W2078903436 date "1981-02-01" @default.
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- W2078903436 title "Automated on-line multi-dimensional high-performance liquid chromatographic techniques for the clean-up and analysis of water-soluble samples" @default.
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- W2078903436 doi "https://doi.org/10.1016/s0021-9673(00)82604-7" @default.
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