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- W2078906089 endingPage "4059" @default.
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- W2078906089 abstract "Antibodies raised against the protein encoded by a lacZ-CDC28 in-frame fusion were shown to immunoprecipitate the CDC28 product from yeast cell lysates. The polypeptide p36CDC28 is a phosphoprotein of apparent Mr 36,000. Immune complexes prepared from yeast cell lysates by using anti-CDC28 antibody were found to possess a protein kinase activity, as determined by the transfer of label from [gamma-32P]ATP to a coprecipitated Mr 40,000 protein of unknown identity or function (p40). This activity was absent or thermolabile when extracts were prepared from several different cdc28 temperature-sensitive strains. The protein kinase activity was dependent on Zn2+ and transferred phosphate specifically to serine and threonine residues." @default.
- W2078906089 created "2016-06-24" @default.
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- W2078906089 date "1985-06-01" @default.
- W2078906089 modified "2023-10-15" @default.
- W2078906089 title "Protein kinase activity associated with the product of the yeast cell division cycle gene CDC28." @default.
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- W2078906089 doi "https://doi.org/10.1073/pnas.82.12.4055" @default.
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