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- W2078907729 abstract "A rapid method for the separation of cytoplasm and nuclei has been adapted for polyamine analysis of the different compartments of BSC-1 cells. The whole cells contain putrescine, spermidine and spermine at concentrations of 7.3, 6.5, and 12.4 nmol/106 cells respectively. By separating the cytoplasm and the nuclei within 20 seconds we found strikingly different polyamine distributions in the cell compartments. In the cytoplasm putrescine and spermine are present in almost equal amounts, whereas in the nucleus spermine is in 5-fold excess over putrescine and spermidine. Fluctuations of polyamines in the different cell compartments were investigated during the cell cycle after synchronizing the cells with thymidine/hydroxyurea treatment. An increase of putrescine in the cytoplasm parallels the initiation of DNA synthesis." @default.
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- W2078907729 date "1982-02-01" @default.
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- W2078907729 title "Compartmentalization of polyamines in mammalian cells" @default.
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- W2078907729 doi "https://doi.org/10.1016/0006-291x(82)91395-x" @default.
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