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• W2078909481 abstract "The single-cell gel (SCG) assay has been shown to be a valuable technique to measure DNA-strand breaks in individual cells. Imaging of the assay is enhanced by laser-scanning microscopy (LSM) technology. Cells embedded in low melt point agarose were treated with 12.5 mM H2O2 to induce DNA-strand breaks. Following cell lysis and electrophoresis under alkaline conditions to allow single-stranded break detection, analysis of the resulting comets can provide an accurate method of comparing DNA-damage levels. The migration patterns of stained DNA were quantitated by LSM analysis. A statistically significant time-dependent dose-response relationship was clearly observed. LSM analysis of the SCG technique allows rapid, sensitive, and reproducible quantitation of single-stranded breaks and alkali-labile sites in the DNA of single cells." @default.
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• W2078909481 date "1993-10-01" @default.
• W2078909481 modified "2023-09-27" @default.
• W2078909481 title "Analysis of single-cell gel electrophoresis using laser-scanning microscopy" @default.
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• W2078909481 doi "https://doi.org/10.1016/0165-1218(93)90071-k" @default.
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