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- W2078931177 abstract "A new sensitive method for adenine nucleotide analysis is described. The key reaction is the phosphorylation of ADP by [32P]PEP in a reaction catalyzed by pyruvate kinase, with the extent of transfer of 32P to ADP being determined by adsorbing the nucleotides onto charcoal. The nonadenine nucleoside diphosphates which also react in the pyruvate kinase reaction are corrected for by determining the 32P retained in the nucleotide fraction after a second incubation with hexokinase and excess glucose. ATP is determined as ADP, after it is quantitatively converted by hexokinase in the presence of excess glucose. Similarly, AMP is analyzed by its conversion to ADP in an incubation with excess ATP and adenylate kinase. The sensitivity of the method for ADP and ATP is 0.05–0.5 pmoles while for AMP it is 5 pmoles." @default.
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- W2078931177 date "1975-11-01" @default.
- W2078931177 modified "2023-09-23" @default.
- W2078931177 title "Analysis of adenine nucleotides at the picomole level with 32P-phosphoenolpyruvate and pyruvate kinase" @default.
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- W2078931177 doi "https://doi.org/10.1016/0003-2697(75)90574-6" @default.
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