Matches in SemOpenAlex for { <https://semopenalex.org/work/W2078952110> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W2078952110 endingPage "86" @default.
- W2078952110 startingPage "79" @default.
- W2078952110 abstract "Six ORFs of unknown function from the left arm of chromosome XII of Saccharomyces cerevisiae were chosen for a reverse genetic approach to provide materials to assist in assignment of function. A two-step PCR using long-flanking homology was employed to amplify disruption cassettes consisting of a kanMX gene as selectable marker flanked by 250-350 bp long regions homologous to the target gene. The diploid strains FY1679 and CEN.PK2 were transformed with the replacement cassettes and transformants were selected for geneticin (G418) resistance. Correct targeting of the replacement cassettes at the genomic locus was verified by Southern blot analysis with the kanMX gene as a probe. Disruption cassettes were cloned in pUG7 plasmid for systematic gene inactivation in other yeast strains and the cognate genes were cloned in pRS416 plasmid for gene complementation studies. Sporulation and tetrad analysis of heterozygous disruptants showed that three of the six ORFs [YLR141w (RRN5), YLR145w and YLR147c (SMD3)] were essential genes that were complemented by their cognate genes. ylr146c Delta (spe4) homozygous diploids showed enhanced sporulation efficiency, whereas ylr147c Delta heterozygous diploids failed to sporulate in the FY1679 but not in the CEN.PK2 genetic background. The other two disruptants [ylr143w and ylr144c (acf2)] gave no phenotype." @default.
- W2078952110 created "2016-06-24" @default.
- W2078952110 creator A5011872049 @default.
- W2078952110 creator A5017412094 @default.
- W2078952110 creator A5028931160 @default.
- W2078952110 creator A5073668335 @default.
- W2078952110 date "2001-01-01" @default.
- W2078952110 modified "2023-09-26" @default.
- W2078952110 title "Disruption of sixSaccharomyces cerevisiae ORFs on chromosome XII results in three lethal disruptants" @default.
- W2078952110 cites W1547269929 @default.
- W2078952110 cites W1549050411 @default.
- W2078952110 cites W1850569493 @default.
- W2078952110 cites W1969498153 @default.
- W2078952110 cites W1996215299 @default.
- W2078952110 cites W2009434837 @default.
- W2078952110 cites W2019377841 @default.
- W2078952110 cites W2032857997 @default.
- W2078952110 cites W2044195710 @default.
- W2078952110 cites W2047439787 @default.
- W2078952110 cites W2080408951 @default.
- W2078952110 cites W2092504070 @default.
- W2078952110 cites W2100057937 @default.
- W2078952110 cites W2108053853 @default.
- W2078952110 cites W2118985834 @default.
- W2078952110 cites W2130292311 @default.
- W2078952110 cites W2140948740 @default.
- W2078952110 cites W2143456837 @default.
- W2078952110 cites W2153283265 @default.
- W2078952110 cites W2166529547 @default.
- W2078952110 cites W2169333620 @default.
- W2078952110 cites W2226935353 @default.
- W2078952110 cites W4240065971 @default.
- W2078952110 doi "https://doi.org/10.1002/yea.808" @default.
- W2078952110 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11754485" @default.
- W2078952110 hasPublicationYear "2001" @default.
- W2078952110 type Work @default.
- W2078952110 sameAs 2078952110 @default.
- W2078952110 citedByCount "1" @default.
- W2078952110 crossrefType "journal-article" @default.
- W2078952110 hasAuthorship W2078952110A5011872049 @default.
- W2078952110 hasAuthorship W2078952110A5017412094 @default.
- W2078952110 hasAuthorship W2078952110A5028931160 @default.
- W2078952110 hasAuthorship W2078952110A5073668335 @default.
- W2078952110 hasConcept C104317684 @default.
- W2078952110 hasConcept C127716648 @default.
- W2078952110 hasConcept C153911025 @default.
- W2078952110 hasConcept C165525559 @default.
- W2078952110 hasConcept C167625842 @default.
- W2078952110 hasConcept C188082640 @default.
- W2078952110 hasConcept C22744801 @default.
- W2078952110 hasConcept C2777576037 @default.
- W2078952110 hasConcept C2780530800 @default.
- W2078952110 hasConcept C30481170 @default.
- W2078952110 hasConcept C42411736 @default.
- W2078952110 hasConcept C47289529 @default.
- W2078952110 hasConcept C54355233 @default.
- W2078952110 hasConcept C84597430 @default.
- W2078952110 hasConcept C86803240 @default.
- W2078952110 hasConceptScore W2078952110C104317684 @default.
- W2078952110 hasConceptScore W2078952110C127716648 @default.
- W2078952110 hasConceptScore W2078952110C153911025 @default.
- W2078952110 hasConceptScore W2078952110C165525559 @default.
- W2078952110 hasConceptScore W2078952110C167625842 @default.
- W2078952110 hasConceptScore W2078952110C188082640 @default.
- W2078952110 hasConceptScore W2078952110C22744801 @default.
- W2078952110 hasConceptScore W2078952110C2777576037 @default.
- W2078952110 hasConceptScore W2078952110C2780530800 @default.
- W2078952110 hasConceptScore W2078952110C30481170 @default.
- W2078952110 hasConceptScore W2078952110C42411736 @default.
- W2078952110 hasConceptScore W2078952110C47289529 @default.
- W2078952110 hasConceptScore W2078952110C54355233 @default.
- W2078952110 hasConceptScore W2078952110C84597430 @default.
- W2078952110 hasConceptScore W2078952110C86803240 @default.
- W2078952110 hasIssue "1" @default.
- W2078952110 hasLocation W20789521101 @default.
- W2078952110 hasLocation W20789521102 @default.
- W2078952110 hasOpenAccess W2078952110 @default.
- W2078952110 hasPrimaryLocation W20789521101 @default.
- W2078952110 hasRelatedWork W2002890816 @default.
- W2078952110 hasRelatedWork W2027674732 @default.
- W2078952110 hasRelatedWork W2030975723 @default.
- W2078952110 hasRelatedWork W2046900167 @default.
- W2078952110 hasRelatedWork W2050573380 @default.
- W2078952110 hasRelatedWork W2050897266 @default.
- W2078952110 hasRelatedWork W2052330886 @default.
- W2078952110 hasRelatedWork W2139665721 @default.
- W2078952110 hasRelatedWork W2152723645 @default.
- W2078952110 hasRelatedWork W4235191842 @default.
- W2078952110 hasVolume "19" @default.
- W2078952110 isParatext "false" @default.
- W2078952110 isRetracted "false" @default.
- W2078952110 magId "2078952110" @default.
- W2078952110 workType "article" @default.