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- W2078960229 abstract "Abstract Hybridization of labelled ribosomal RNA of Bacillus subtilis Marburg strain with DNA's from various microorganisms was studied by means of a DNA-agar method involving ribonuclease digestion. The size and base ratio of the hybridized rRNA portion were analyzed. The B. subtilis rRNA was found to be highly complementary to the DNA's of Bacilli tested: the extent of the analogy seemed to be correlated with the gehetic relatedness and the guanine-cytosine content of the DNA. Appreciable cross-hybridization was also observed between B. subtilis rRNA and DNA from microorganisms of different families, e.g. the extents of hybridization with DNA's from Staphylococcus epidermidis, Alcaligenes faecalis, and Escherichia coli were 55, 33 and 20 %, respectively, of that with the homologous DNA. Competition experiments revealed similar extents of the analogy of rRNA in these bacteria. In addition, the heterologous hybridization between Staphylococcus DNA and labelled rRNA from B. subtilis was reduced by unlabelled rRNA from A. faecalis by the expected amount. The results suggest that analogous base sequences may occur at the same site(s) in the rRNA's from these bacteria. The majority of experiments were carried out with 23-S RNA, but similar results were also obtained with 16-S RNA. These results suggest the conservation of rRNA sites in the DNA of microorganisms." @default.
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- W2078960229 date "1967-01-01" @default.
- W2078960229 modified "2023-10-18" @default.
- W2078960229 title "Species specificity of the ribosomal RNA cistrons in bacteria" @default.
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- W2078960229 doi "https://doi.org/10.1016/0005-2787(67)90095-0" @default.
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