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• W2078984957 abstract "Peptide fragments corresponding to the N- and C-terminal portions of bovine ubiquitin, U(1−35) and U(36−76), are shown by NMR to associate in solution to form a complex of modest stability (Kassn ≈ 1.4 × 105 M-1 at pH 7.0), with NMR features characteristic of a nativelike structure. The complex undergoes cold denaturation, with temperature-dependent estimates of stability from NMR indicating a ΔCp° for fragment complexation in good agreement with that determined for native ubiquitin, suggesting that fragment association results in the burial of a similar hydrophobic surface area. The stability of the complex shows appreciable pH dependence, suggesting that ionic interactions on the surface of the protein contribute significantly. However, denaturation studies of native ubiquitin in the presence of guanidine hydrochloride (Gdn·HCl) show little pH dependence, suggesting that ionic interactions may be “screened” by the denaturant, as recently suggested. Examination of the conformation of the isolated peptide fragments has shown evidence for a low population of nativelike structure in the N-terminal β-hairpin (residues 1−17) and weak nascent helical propensity in the helical fragment (residues 21−35). In contrast, the C-terminal peptide (36−76) shows evidence in aqueous solution, from some Hα chemical shifts, for nonnative φ and ψ angles; nonnative α-helical structure is readily induced in the presence of organic cosolvents, indicating that tertiary interactions in both native ubiquitin and the folded fragment complex strongly dictate its structural preference. The data suggest that the N-terminal fragment (1−35), where interaction between the helix and hairpin requires the minimum loss of conformational entropy, may provide the nucleation site for fragment complexation." @default.
• W2078984957 created "2016-06-24" @default.
• W2078984957 creator A5060134936 @default.
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• W2078984957 date "2000-09-12" @default.
• W2078984957 modified "2023-09-30" @default.
• W2078984957 title "Cooperative Assembly of a Nativelike Ubiquitin Structure through Peptide Fragment Complexation: Energetics of Peptide Association and Folding" @default.
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• W2078984957 doi "https://doi.org/10.1021/bi000718r" @default.
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