Matches in SemOpenAlex for { <https://semopenalex.org/work/W2078986709> ?p ?o ?g. }
Showing items 1 to 88 of
88
with 100 items per page.
- W2078986709 endingPage "277" @default.
- W2078986709 startingPage "271" @default.
- W2078986709 abstract "The aim of the present study was the development of a genetic discrimination method named Melt-Curve-MHS-PCR (MC-MHS-PCR). In order to explore the advantages, limitations and designing aspects of this method, we have used the conventional (electrophoretic) MHS-PCR as a reference, and designed species-discrimination assays for three fishes of the genus Spicara: Spicara smaris, Spicara maena and Spicara flexuosa. These small-size fishes exhibit marked phenotypic diversity that hinders their discrimination. Partial 16S rRNA gene sequences were obtained from twelve Mediterranean Spicara individuals and were used for the designing of two primer-sets that were subsequently tested on Spicara specimens from six sampling locations of Eastern Mediterranean. All examined samples were correctly assigned by both conventional and melt-curve MHS-PCR, proving that the MC-MHS-PCR is as reliable and discriminatory as the MHS-PCR. Moreover, the MC-MHS-PCR is very rapid, easily implemented, straightforward, and minimizes the risk of sample mix-up and laboratory contamination. These features render the method suitable for large-scale discrimination tests, particularly for studies that include small number of species." @default.
- W2078986709 created "2016-06-24" @default.
- W2078986709 creator A5011271208 @default.
- W2078986709 creator A5024626594 @default.
- W2078986709 creator A5028913501 @default.
- W2078986709 creator A5079093785 @default.
- W2078986709 date "2014-10-01" @default.
- W2078986709 modified "2023-10-18" @default.
- W2078986709 title "Melt-curve-multiplex-haplotype-specific-PCR, a valuable tool for biological studies: Application in congeneric species discrimination assay" @default.
- W2078986709 cites W1981333527 @default.
- W2078986709 cites W2002201987 @default.
- W2078986709 cites W2016426200 @default.
- W2078986709 cites W2017711206 @default.
- W2078986709 cites W2028166468 @default.
- W2078986709 cites W2053168292 @default.
- W2078986709 cites W2060006075 @default.
- W2078986709 cites W2066137273 @default.
- W2078986709 cites W2078903059 @default.
- W2078986709 cites W2085975673 @default.
- W2078986709 cites W2088510827 @default.
- W2078986709 cites W2093143496 @default.
- W2078986709 cites W2101819429 @default.
- W2078986709 cites W2103170384 @default.
- W2078986709 cites W2128126965 @default.
- W2078986709 cites W2147441182 @default.
- W2078986709 cites W2157982958 @default.
- W2078986709 cites W2158388229 @default.
- W2078986709 cites W2159219251 @default.
- W2078986709 cites W2162229053 @default.
- W2078986709 cites W2166197562 @default.
- W2078986709 cites W2471379004 @default.
- W2078986709 doi "https://doi.org/10.1016/j.bse.2014.07.013" @default.
- W2078986709 hasPublicationYear "2014" @default.
- W2078986709 type Work @default.
- W2078986709 sameAs 2078986709 @default.
- W2078986709 citedByCount "1" @default.
- W2078986709 countsByYear W20789867092021 @default.
- W2078986709 crossrefType "journal-article" @default.
- W2078986709 hasAuthorship W2078986709A5011271208 @default.
- W2078986709 hasAuthorship W2078986709A5024626594 @default.
- W2078986709 hasAuthorship W2078986709A5028913501 @default.
- W2078986709 hasAuthorship W2078986709A5079093785 @default.
- W2078986709 hasConcept C104317684 @default.
- W2078986709 hasConcept C106131492 @default.
- W2078986709 hasConcept C140779682 @default.
- W2078986709 hasConcept C178790620 @default.
- W2078986709 hasConcept C185592680 @default.
- W2078986709 hasConcept C2777563447 @default.
- W2078986709 hasConcept C31972630 @default.
- W2078986709 hasConcept C41008148 @default.
- W2078986709 hasConcept C49105822 @default.
- W2078986709 hasConcept C54355233 @default.
- W2078986709 hasConcept C70721500 @default.
- W2078986709 hasConcept C86803240 @default.
- W2078986709 hasConcept C90583042 @default.
- W2078986709 hasConceptScore W2078986709C104317684 @default.
- W2078986709 hasConceptScore W2078986709C106131492 @default.
- W2078986709 hasConceptScore W2078986709C140779682 @default.
- W2078986709 hasConceptScore W2078986709C178790620 @default.
- W2078986709 hasConceptScore W2078986709C185592680 @default.
- W2078986709 hasConceptScore W2078986709C2777563447 @default.
- W2078986709 hasConceptScore W2078986709C31972630 @default.
- W2078986709 hasConceptScore W2078986709C41008148 @default.
- W2078986709 hasConceptScore W2078986709C49105822 @default.
- W2078986709 hasConceptScore W2078986709C54355233 @default.
- W2078986709 hasConceptScore W2078986709C70721500 @default.
- W2078986709 hasConceptScore W2078986709C86803240 @default.
- W2078986709 hasConceptScore W2078986709C90583042 @default.
- W2078986709 hasLocation W20789867091 @default.
- W2078986709 hasOpenAccess W2078986709 @default.
- W2078986709 hasPrimaryLocation W20789867091 @default.
- W2078986709 hasRelatedWork W169075148 @default.
- W2078986709 hasRelatedWork W1966568994 @default.
- W2078986709 hasRelatedWork W2042343285 @default.
- W2078986709 hasRelatedWork W2072460497 @default.
- W2078986709 hasRelatedWork W2080534576 @default.
- W2078986709 hasRelatedWork W2158767261 @default.
- W2078986709 hasRelatedWork W2247793475 @default.
- W2078986709 hasRelatedWork W2391953454 @default.
- W2078986709 hasRelatedWork W2403090579 @default.
- W2078986709 hasRelatedWork W2462933426 @default.
- W2078986709 hasVolume "56" @default.
- W2078986709 isParatext "false" @default.
- W2078986709 isRetracted "false" @default.
- W2078986709 magId "2078986709" @default.
- W2078986709 workType "article" @default.