Matches in SemOpenAlex for { <https://semopenalex.org/work/W207899471> ?p ?o ?g. }
Showing items 1 to 72 of
72
with 100 items per page.
- W207899471 endingPage "65" @default.
- W207899471 startingPage "58" @default.
- W207899471 abstract "Monoclonal antibodies specific to human prolactin were generated by an improved hybridoma technique. Following immunization with hormone conjugated with hemocyanin and cell fusion, hybrid cells were first cultured in a semisolid medium containing methylcellulose and later transferred to liquid medium for subculture. Out of 1500 colonies that were initially recovered in one single cell fusion experiment, 300 were shown to exhibit affinity to human prolactin by enzyme-linked immunosorbent assay and radioimmunoassay. Finally, nine monoclonal antibodies having high affinity and specificity to human prolactin were selected for further evaluations. From the results of a cross-matching procedure, one pair of antibodies reacting with discrete antigenic determinants of human prolactin was identified. Using a pair of monoclonal antibodies, the solid phase sandwich immunoradiometric assay and enzyme immunoassay were designed. The sensitivity of these 1-h immunoassay procedures for the determination of human prolactin was 2 ng/ml." @default.
- W207899471 created "2016-06-24" @default.
- W207899471 creator A5031219952 @default.
- W207899471 creator A5039896345 @default.
- W207899471 creator A5041428329 @default.
- W207899471 creator A5079745721 @default.
- W207899471 date "1987-02-01" @default.
- W207899471 modified "2023-09-23" @default.
- W207899471 title "Generation of monoclonal antibodies to human prolactin and applications in solid-phase immunoassays." @default.
- W207899471 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3566920" @default.
- W207899471 hasPublicationYear "1987" @default.
- W207899471 type Work @default.
- W207899471 sameAs 207899471 @default.
- W207899471 citedByCount "0" @default.
- W207899471 crossrefType "journal-article" @default.
- W207899471 hasAuthorship W207899471A5031219952 @default.
- W207899471 hasAuthorship W207899471A5039896345 @default.
- W207899471 hasAuthorship W207899471A5041428329 @default.
- W207899471 hasAuthorship W207899471A5079745721 @default.
- W207899471 hasConcept C1491633281 @default.
- W207899471 hasConcept C153911025 @default.
- W207899471 hasConcept C159654299 @default.
- W207899471 hasConcept C163864487 @default.
- W207899471 hasConcept C176685330 @default.
- W207899471 hasConcept C185592680 @default.
- W207899471 hasConcept C203014093 @default.
- W207899471 hasConcept C2778758028 @default.
- W207899471 hasConcept C2779064019 @default.
- W207899471 hasConcept C2780420688 @default.
- W207899471 hasConcept C43617362 @default.
- W207899471 hasConcept C542903549 @default.
- W207899471 hasConcept C55493867 @default.
- W207899471 hasConcept C71315377 @default.
- W207899471 hasConcept C86803240 @default.
- W207899471 hasConcept C92488253 @default.
- W207899471 hasConceptScore W207899471C1491633281 @default.
- W207899471 hasConceptScore W207899471C153911025 @default.
- W207899471 hasConceptScore W207899471C159654299 @default.
- W207899471 hasConceptScore W207899471C163864487 @default.
- W207899471 hasConceptScore W207899471C176685330 @default.
- W207899471 hasConceptScore W207899471C185592680 @default.
- W207899471 hasConceptScore W207899471C203014093 @default.
- W207899471 hasConceptScore W207899471C2778758028 @default.
- W207899471 hasConceptScore W207899471C2779064019 @default.
- W207899471 hasConceptScore W207899471C2780420688 @default.
- W207899471 hasConceptScore W207899471C43617362 @default.
- W207899471 hasConceptScore W207899471C542903549 @default.
- W207899471 hasConceptScore W207899471C55493867 @default.
- W207899471 hasConceptScore W207899471C71315377 @default.
- W207899471 hasConceptScore W207899471C86803240 @default.
- W207899471 hasConceptScore W207899471C92488253 @default.
- W207899471 hasIssue "1" @default.
- W207899471 hasLocation W2078994711 @default.
- W207899471 hasOpenAccess W207899471 @default.
- W207899471 hasPrimaryLocation W2078994711 @default.
- W207899471 hasRelatedWork W1943797027 @default.
- W207899471 hasRelatedWork W1974139039 @default.
- W207899471 hasRelatedWork W1992169430 @default.
- W207899471 hasRelatedWork W1998831848 @default.
- W207899471 hasRelatedWork W2067505685 @default.
- W207899471 hasRelatedWork W207899471 @default.
- W207899471 hasRelatedWork W2145860954 @default.
- W207899471 hasRelatedWork W2361872411 @default.
- W207899471 hasRelatedWork W5602368 @default.
- W207899471 hasRelatedWork W4290419591 @default.
- W207899471 hasVolume "9" @default.
- W207899471 isParatext "false" @default.
- W207899471 isRetracted "false" @default.
- W207899471 magId "207899471" @default.
- W207899471 workType "article" @default.