Matches in SemOpenAlex for { <https://semopenalex.org/work/W2078996419> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W2078996419 endingPage "3622" @default.
- W2078996419 startingPage "3615" @default.
- W2078996419 abstract "We have systematically designed and synthesized six kinds of 16-17 mer alanine-based peptides containing four to six lysine (K) and one to four asparagine (N) residues to achieve the selective binding to A.T base pairs of DNA duplexes. The position and number of K and N residues were changed in the helical structure according to common features of the DNA-binding proteins, in which K and N residues are expected to interact electrostatically with phosphate groups and to interact with A.T base pairs by hydrogen bonding, respectively. The time courses of binding of these peptides to dA(30).dT(30) and dG(30).dC(30) duplexes immobilized on a 27 MHz quartz crystal microbalance (QCM) were studied in 10 mM phosphate buffer (pH 7.5) and 40 mM NaCl at 10 degrees C. The maximum binding amounts (Deltam(max)) on a nanogram scale and binding constants (K(a)) could be obtained from the frequency decrease (mass increase) of the oligonucleotide-immobilized QCM. The conformation changes of the peptides upon binding to DNAs were monitored by circular dichroism (CD) spectroscopy. The four properly arranged N residues in the six-cationic K peptide, K6N4(d), resulted in a 5-fold higher affinity for A.T base pairs (K(a) = 5.9 x 10(5) M(-1)) than for G.C base pairs (K(a) = 1.2 x 10(5) M(-1)), and alpha-helices were clearly promoted by the binding to A.T base pairs from CD spectral changes." @default.
- W2078996419 created "2016-06-24" @default.
- W2078996419 creator A5001611564 @default.
- W2078996419 creator A5032135459 @default.
- W2078996419 creator A5088195507 @default.
- W2078996419 date "2001-03-01" @default.
- W2078996419 modified "2023-09-24" @default.
- W2078996419 title "Design and Characterization of Asparagine- and Lysine-Containing Alanine-Based Helical Peptides That Bind Selectively to A·T Base Pairs of Oligonucleotides Immobilized on a 27 MHz Quartz Crystal Microbalance" @default.
- W2078996419 cites W1501052785 @default.
- W2078996419 cites W1986276257 @default.
- W2078996419 cites W1988014923 @default.
- W2078996419 cites W2011131418 @default.
- W2078996419 cites W2013715518 @default.
- W2078996419 cites W2014655418 @default.
- W2078996419 cites W2019070778 @default.
- W2078996419 cites W2029313236 @default.
- W2078996419 cites W2047973051 @default.
- W2078996419 cites W2056115277 @default.
- W2078996419 cites W2060330087 @default.
- W2078996419 cites W2126886323 @default.
- W2078996419 cites W2132185105 @default.
- W2078996419 cites W2153805912 @default.
- W2078996419 cites W2796940875 @default.
- W2078996419 cites W50469311 @default.
- W2078996419 doi "https://doi.org/10.1021/bi001699o" @default.
- W2078996419 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11297428" @default.
- W2078996419 hasPublicationYear "2001" @default.
- W2078996419 type Work @default.
- W2078996419 sameAs 2078996419 @default.
- W2078996419 citedByCount "42" @default.
- W2078996419 countsByYear W20789964192012 @default.
- W2078996419 countsByYear W20789964192013 @default.
- W2078996419 countsByYear W20789964192014 @default.
- W2078996419 countsByYear W20789964192017 @default.
- W2078996419 crossrefType "journal-article" @default.
- W2078996419 hasAuthorship W2078996419A5001611564 @default.
- W2078996419 hasAuthorship W2078996419A5032135459 @default.
- W2078996419 hasAuthorship W2078996419A5088195507 @default.
- W2078996419 hasConcept C129312508 @default.
- W2078996419 hasConcept C133571119 @default.
- W2078996419 hasConcept C150394285 @default.
- W2078996419 hasConcept C178790620 @default.
- W2078996419 hasConcept C185581394 @default.
- W2078996419 hasConcept C185592680 @default.
- W2078996419 hasConcept C2775895851 @default.
- W2078996419 hasConcept C2776016237 @default.
- W2078996419 hasConcept C2779281246 @default.
- W2078996419 hasConcept C2779856020 @default.
- W2078996419 hasConcept C515207424 @default.
- W2078996419 hasConcept C552990157 @default.
- W2078996419 hasConcept C55493867 @default.
- W2078996419 hasConcept C71240020 @default.
- W2078996419 hasConcept C8010536 @default.
- W2078996419 hasConcept C9996572 @default.
- W2078996419 hasConceptScore W2078996419C129312508 @default.
- W2078996419 hasConceptScore W2078996419C133571119 @default.
- W2078996419 hasConceptScore W2078996419C150394285 @default.
- W2078996419 hasConceptScore W2078996419C178790620 @default.
- W2078996419 hasConceptScore W2078996419C185581394 @default.
- W2078996419 hasConceptScore W2078996419C185592680 @default.
- W2078996419 hasConceptScore W2078996419C2775895851 @default.
- W2078996419 hasConceptScore W2078996419C2776016237 @default.
- W2078996419 hasConceptScore W2078996419C2779281246 @default.
- W2078996419 hasConceptScore W2078996419C2779856020 @default.
- W2078996419 hasConceptScore W2078996419C515207424 @default.
- W2078996419 hasConceptScore W2078996419C552990157 @default.
- W2078996419 hasConceptScore W2078996419C55493867 @default.
- W2078996419 hasConceptScore W2078996419C71240020 @default.
- W2078996419 hasConceptScore W2078996419C8010536 @default.
- W2078996419 hasConceptScore W2078996419C9996572 @default.
- W2078996419 hasIssue "12" @default.
- W2078996419 hasLocation W20789964191 @default.
- W2078996419 hasLocation W20789964192 @default.
- W2078996419 hasOpenAccess W2078996419 @default.
- W2078996419 hasPrimaryLocation W20789964191 @default.
- W2078996419 hasRelatedWork W2005783693 @default.
- W2078996419 hasRelatedWork W2033358700 @default.
- W2078996419 hasRelatedWork W2047879163 @default.
- W2078996419 hasRelatedWork W2051801995 @default.
- W2078996419 hasRelatedWork W2073760869 @default.
- W2078996419 hasRelatedWork W2078996419 @default.
- W2078996419 hasRelatedWork W2126886323 @default.
- W2078996419 hasRelatedWork W2394841584 @default.
- W2078996419 hasRelatedWork W2395952657 @default.
- W2078996419 hasRelatedWork W2792490921 @default.
- W2078996419 hasVolume "40" @default.
- W2078996419 isParatext "false" @default.
- W2078996419 isRetracted "false" @default.
- W2078996419 magId "2078996419" @default.
- W2078996419 workType "article" @default.