Matches in SemOpenAlex for { <https://semopenalex.org/work/W2079003283> ?p ?o ?g. }
- W2079003283 endingPage "1076" @default.
- W2079003283 startingPage "1071" @default.
- W2079003283 abstract "We developed an ultracentrifugation and high-performance liquid chromatography (HPLC) method for simultaneous measurement of cholesterol in serum high density lipoprotein (HDL) and low density lipoprotein (LDL) subfractions and lipoprotein (a) [Lp(a)]. Serum aliquots of 0.05 ml were centrifuged at background densities of 1.006, 1.044 kg/l, and in the presence of β-mercaptoethanol (ME) at background densities of 1.044, 1.063 and 1.125 kg/l for the separation of lipoprotein subfractions and Lp(a). Cholesterol levels in the ultracentrifugal bottom fractions were analyzed by HPLC. ME effectively dissociated Lp(a) into apolipoprotein (a) and Lp(a) remnant [Lp(a-)]. Lp(a-) showed a distinctive density distribution from that of the native Lp(a). Based on these data, a method was developed to separate lipoprotein into subfractions and Lp(a) by ultracentrifugation. The separated HDL and LDL subfractions were not contaminated with Lp(a). This method is highly precise with the total CVs for the measurement of HDL2-C, HDL3-C, LDLa-C, LDLb-C and Lp(a)-C 0.85%–2.66%, 0.87%–3.21%, 0.86%–1.11%, 2.59%–6.35% and 4.42%–12.29%, respectively. A new method for the separation of HDL and LDL subfractions and Lp(a) and simultaneous measurement of cholesterol by ultracentrifugation and HPLC have been established. It is precise and sensitive and can be used in research or clinical laboratories for lipoprotein profiling." @default.
- W2079003283 created "2016-06-24" @default.
- W2079003283 creator A5011894823 @default.
- W2079003283 creator A5013357777 @default.
- W2079003283 creator A5026787386 @default.
- W2079003283 creator A5041324566 @default.
- W2079003283 creator A5042772797 @default.
- W2079003283 creator A5059150167 @default.
- W2079003283 creator A5077837300 @default.
- W2079003283 creator A5090918351 @default.
- W2079003283 date "2012-07-01" @default.
- W2079003283 modified "2023-10-09" @default.
- W2079003283 title "A novel and precise method for simultaneous measurement of serum HDL and LDL subfractions and lipoprotein (a) cholesterol by ultracentrifugation and high-performance liquid chromatography" @default.
- W2079003283 cites W1486434785 @default.
- W2079003283 cites W1575753161 @default.
- W2079003283 cites W1580303209 @default.
- W2079003283 cites W1891714429 @default.
- W2079003283 cites W1902354643 @default.
- W2079003283 cites W1907534963 @default.
- W2079003283 cites W1972440654 @default.
- W2079003283 cites W1996810831 @default.
- W2079003283 cites W1998563250 @default.
- W2079003283 cites W2004565409 @default.
- W2079003283 cites W2008369450 @default.
- W2079003283 cites W2022756739 @default.
- W2079003283 cites W2038613206 @default.
- W2079003283 cites W2047303917 @default.
- W2079003283 cites W2059400200 @default.
- W2079003283 cites W2063796082 @default.
- W2079003283 cites W2095471511 @default.
- W2079003283 cites W2107818754 @default.
- W2079003283 cites W2129041722 @default.
- W2079003283 cites W2141712507 @default.
- W2079003283 cites W2142103691 @default.
- W2079003283 cites W2165076848 @default.
- W2079003283 cites W2169236009 @default.
- W2079003283 cites W2184549447 @default.
- W2079003283 cites W2185919342 @default.
- W2079003283 cites W2189623052 @default.
- W2079003283 cites W2915324964 @default.
- W2079003283 cites W4230870013 @default.
- W2079003283 doi "https://doi.org/10.1016/j.cca.2012.02.022" @default.
- W2079003283 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22406178" @default.
- W2079003283 hasPublicationYear "2012" @default.
- W2079003283 type Work @default.
- W2079003283 sameAs 2079003283 @default.
- W2079003283 citedByCount "11" @default.
- W2079003283 countsByYear W20790032832013 @default.
- W2079003283 countsByYear W20790032832014 @default.
- W2079003283 countsByYear W20790032832015 @default.
- W2079003283 countsByYear W20790032832017 @default.
- W2079003283 countsByYear W20790032832019 @default.
- W2079003283 countsByYear W20790032832020 @default.
- W2079003283 countsByYear W20790032832023 @default.
- W2079003283 crossrefType "journal-article" @default.
- W2079003283 hasAuthorship W2079003283A5011894823 @default.
- W2079003283 hasAuthorship W2079003283A5013357777 @default.
- W2079003283 hasAuthorship W2079003283A5026787386 @default.
- W2079003283 hasAuthorship W2079003283A5041324566 @default.
- W2079003283 hasAuthorship W2079003283A5042772797 @default.
- W2079003283 hasAuthorship W2079003283A5059150167 @default.
- W2079003283 hasAuthorship W2079003283A5077837300 @default.
- W2079003283 hasAuthorship W2079003283A5090918351 @default.
- W2079003283 hasConcept C179998833 @default.
- W2079003283 hasConcept C185592680 @default.
- W2079003283 hasConcept C2776104379 @default.
- W2079003283 hasConcept C2778163477 @default.
- W2079003283 hasConcept C2780072125 @default.
- W2079003283 hasConcept C2780541478 @default.
- W2079003283 hasConcept C2910425616 @default.
- W2079003283 hasConcept C43617362 @default.
- W2079003283 hasConcept C54689828 @default.
- W2079003283 hasConcept C55493867 @default.
- W2079003283 hasConcept C62746215 @default.
- W2079003283 hasConcept C7623868 @default.
- W2079003283 hasConceptScore W2079003283C179998833 @default.
- W2079003283 hasConceptScore W2079003283C185592680 @default.
- W2079003283 hasConceptScore W2079003283C2776104379 @default.
- W2079003283 hasConceptScore W2079003283C2778163477 @default.
- W2079003283 hasConceptScore W2079003283C2780072125 @default.
- W2079003283 hasConceptScore W2079003283C2780541478 @default.
- W2079003283 hasConceptScore W2079003283C2910425616 @default.
- W2079003283 hasConceptScore W2079003283C43617362 @default.
- W2079003283 hasConceptScore W2079003283C54689828 @default.
- W2079003283 hasConceptScore W2079003283C55493867 @default.
- W2079003283 hasConceptScore W2079003283C62746215 @default.
- W2079003283 hasConceptScore W2079003283C7623868 @default.
- W2079003283 hasFunder F4320321001 @default.
- W2079003283 hasIssue "13-14" @default.
- W2079003283 hasLocation W20790032831 @default.
- W2079003283 hasLocation W20790032832 @default.
- W2079003283 hasOpenAccess W2079003283 @default.
- W2079003283 hasPrimaryLocation W20790032831 @default.
- W2079003283 hasRelatedWork W2014078720 @default.
- W2079003283 hasRelatedWork W2020286373 @default.
- W2079003283 hasRelatedWork W2020629107 @default.
- W2079003283 hasRelatedWork W2028271503 @default.
- W2079003283 hasRelatedWork W2079003283 @default.