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• W2079013412 abstract "Mucosal explants were prepared from the colons of normal rats and from the non-neoplastic colonic mucosa of rats which had been treated chronically with the intestinal carcinogen dimethylhydrazine. They were maintained in an organ culture system which permitted survival up to at least 25 days. Morphological preservation of the mucosa was excellent up to 6 days in culture and thereafter changes began to occur. But even at 25 days normal crypt structures were still evident. The hyperplastic and dysplastic changes seen in pre-culture samples of DMH-treated mucosae remained recognisable during the first two days in culture. They were no longer seen in explants examined after this time however and, indeed, there appeared to be no difference in the morphology and survival of control and DMH-treated mucosae. It is possible that our culture system does not permit further neoplastic progression, but an alternative explanation is that the system discriminates specifically against the survival of neoplastic elements." @default.
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• W2079013412 date "1984-03-01" @default.
• W2079013412 modified "2023-10-10" @default.
• W2079013412 title "Morphological studies on the long-term organ culture of colonic mucosa from normal and dimethylhydrazine treated rats" @default.
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• W2079013412 doi "https://doi.org/10.1038/bjc.1984.45" @default.
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