FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2079025500> ?p ?o ?g. }

• W2079025500 abstract "Abstract Background Aspergillus fumigatus is a thermotolerant human-pathogenic mold and the most common cause of invasive aspergillosis (IA) in immunocompromised patients. Its predominance is based on several factors most of which are still unknown. The thermotolerance of A. fumigatus is one of the traits which have been assigned to pathogenicity. It allows the fungus to grow at temperatures up to and above that of a fevered human host. To elucidate the mechanisms of heat resistance, we analyzed the change of the A. fumigatus proteome during a temperature shift from 30°C to 48°C by 2D-fluorescence difference gel electrophoresis (DIGE). To improve 2D gel image analysis results, protein spot quantitation was optimized by missing value imputation and normalization. Differentially regulated proteins were compared to previously published transcriptome data of A. fumigatus . The study was augmented by bioinformatical analysis of transcription factor binding sites (TFBSs) in the promoter region of genes whose corresponding proteins were differentially regulated upon heat shock. Results 91 differentially regulated protein spots, representing 64 different proteins, were identified by mass spectrometry (MS). They showed a continuous up-, down- or an oscillating regulation. Many of the identified proteins were involved in protein folding (chaperones), oxidative stress response, signal transduction, transcription, translation, carbohydrate and nitrogen metabolism. A correlation between alteration of transcript levels and corresponding proteins was detected for half of the differentially regulated proteins. Interestingly, some previously undescribed putative targets for the heat shock regulator Hsf1 were identified. This provides evidence for Hsf1-dependent regulation of mannitol biosynthesis, translation, cytoskeletal dynamics and cell division in A. fumigatus . Furthermore, computational analysis of promoters revealed putative binding sites for an AP-2alpha-like transcription factor upstream of some heat shock induced genes. Until now, this factor has only been found in vertebrates. Conclusions Our newly established DIGE data analysis workflow yields improved data quality and is widely applicable for other DIGE datasets. Our findings suggest that the heat shock response in A. fumigatus differs from already well-studied yeasts and other filamentous fungi." @default.
• W2079025500 created "2016-06-24" @default.
• W2079025500 creator A5000809255 @default.
• W2079025500 creator A5040372260 @default.
• W2079025500 creator A5054532448 @default.
• W2079025500 creator A5071982932 @default.
• W2079025500 date "2010-01-15" @default.
• W2079025500 modified "2023-10-13" @default.
• W2079025500 title "Integrative analysis of the heat shock response in Aspergillus fumigatus" @default.
• W2079025500 cites W146632794 @default.
• W2079025500 cites W1860599747 @default.
• W2079025500 cites W1930219409 @default.
• W2079025500 cites W1964313477 @default.
• W2079025500 cites W1971286829 @default.
• W2079025500 cites W1976704049 @default.
• W2079025500 cites W1980645672 @default.
• W2079025500 cites W1985447576 @default.
• W2079025500 cites W1987531919 @default.
• W2079025500 cites W1987544400 @default.
• W2079025500 cites W1991456063 @default.
• W2079025500 cites W1992097460 @default.
• W2079025500 cites W1992824986 @default.
• W2079025500 cites W1994483281 @default.
• W2079025500 cites W1994854020 @default.
• W2079025500 cites W2004211776 @default.
• W2079025500 cites W2004254720 @default.
• W2079025500 cites W2009497024 @default.
• W2079025500 cites W2011572906 @default.
• W2079025500 cites W2013803609 @default.
• W2079025500 cites W2020854337 @default.
• W2079025500 cites W2021575974 @default.
• W2079025500 cites W2037846837 @default.
• W2079025500 cites W2037992536 @default.
• W2079025500 cites W2052237198 @default.
• W2079025500 cites W2053403241 @default.
• W2079025500 cites W2056006751 @default.
• W2079025500 cites W2061518309 @default.
• W2079025500 cites W2065992894 @default.
• W2079025500 cites W2070066454 @default.
• W2079025500 cites W2073136670 @default.
• W2079025500 cites W2074529937 @default.
• W2079025500 cites W2075241233 @default.
• W2079025500 cites W2076794100 @default.
• W2079025500 cites W2076981817 @default.
• W2079025500 cites W2095542431 @default.
• W2079025500 cites W2099170652 @default.
• W2079025500 cites W2106326652 @default.
• W2079025500 cites W2107363136 @default.
• W2079025500 cites W2108185386 @default.
• W2079025500 cites W2108313755 @default.
• W2079025500 cites W2111060309 @default.
• W2079025500 cites W2119941231 @default.
• W2079025500 cites W2122436557 @default.
• W2079025500 cites W2124551605 @default.
• W2079025500 cites W2125079066 @default.
• W2079025500 cites W2126024920 @default.
• W2079025500 cites W2127754721 @default.
• W2079025500 cites W2129331994 @default.
• W2079025500 cites W2134809769 @default.
• W2079025500 cites W2135752097 @default.
• W2079025500 cites W2137683543 @default.
• W2079025500 cites W2137917513 @default.
• W2079025500 cites W2141148930 @default.
• W2079025500 cites W2143129697 @default.
• W2079025500 cites W2143287609 @default.
• W2079025500 cites W2143727851 @default.
• W2079025500 cites W2145091349 @default.
• W2079025500 cites W2147104869 @default.
• W2079025500 cites W2147440610 @default.
• W2079025500 cites W2149064463 @default.
• W2079025500 cites W2157163471 @default.
• W2079025500 cites W2160167196 @default.
• W2079025500 cites W2165008731 @default.
• W2079025500 cites W2166884187 @default.
• W2079025500 cites W2166953436 @default.
• W2079025500 cites W2168656346 @default.
• W2079025500 cites W2170949754 @default.
• W2079025500 cites W4210608161 @default.
• W2079025500 cites W4293247451 @default.
• W2079025500 cites W2151563713 @default.
• W2079025500 doi "https://doi.org/10.1186/1471-2164-11-32" @default.
• W2079025500 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2820008" @default.
• W2079025500 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20074381" @default.
• W2079025500 hasPublicationYear "2010" @default.
• W2079025500 type Work @default.
• W2079025500 sameAs 2079025500 @default.
• W2079025500 citedByCount "76" @default.
• W2079025500 countsByYear W20790255002012 @default.
• W2079025500 countsByYear W20790255002013 @default.
• W2079025500 countsByYear W20790255002014 @default.
• W2079025500 countsByYear W20790255002015 @default.
• W2079025500 countsByYear W20790255002016 @default.
• W2079025500 countsByYear W20790255002017 @default.
• W2079025500 countsByYear W20790255002018 @default.
• W2079025500 countsByYear W20790255002019 @default.
• W2079025500 countsByYear W20790255002020 @default.
• W2079025500 countsByYear W20790255002021 @default.
• W2079025500 countsByYear W20790255002022 @default.
• W2079025500 countsByYear W20790255002023 @default.
• W2079025500 crossrefType "journal-article" @default.

• next