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- W2079051679 abstract "Cycloartenol synthase converts oxidosqualene to the pentacyclic sterol precursor cycloartenol. An Arabidopsis thaliana cycloartenol synthase Ile481Val mutant was previously shown to produce lanosterol and parkeol in addition to its native product cycloartenol. Experiments are described here to construct Phe, Leu, Ala, and Gly mutants at position 481 and to determine their cyclization product profiles. The Phe mutant was inactive, and the Leu mutant produced cycloartenol and parkeol. The Ala and Gly mutants formed lanosterol, cycloartenol, parkeol, achilleol A, and camelliol C. Monocycles comprise most of the Gly mutant product, showing that an alternate cyclization route can be made the major pathway by a single nonpolar mutation." @default.
- W2079051679 created "2016-06-24" @default.
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- W2079051679 date "2000-07-01" @default.
- W2079051679 modified "2023-10-18" @default.
- W2079051679 title "Steric Bulk at Cycloartenol Synthase Position 481 Influences Cyclization and Deprotonation" @default.
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- W2079051679 doi "https://doi.org/10.1021/ol006018w" @default.
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