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• W2079056064 abstract "Preparative high-speed counter-current chromatography (HSCCC) was successfully applied to the isolation and purification of three stilbene oligomers from Vitis chunganeniss using stepwise elution with a pair of two-phase solvent systems composed of n-hexane–ethyl acetate–methanol–water at (2:5:2:5, v/v) and (1:2:1:2, v/v). The preparative HSCCC separation was performed on 800 mg of crude sample yielding hopeaphenol (21.1 mg), amurensin G (37.2 mg) and vitisin A (95.6 mg) in a one-step separation, with purities over 95% as determined by HPLC. The structures of these three compounds were identified by MS, 1H NMR and 13C NMR. In addition, their antioxidant activities were screened by DPPH assay, where vitisin A showed strong antioxidant activity. Further EPR experiments with spin-trapping technique demonstrated that vitisin A is a potent and selective singlet oxygen quencher, which may be used in singlet oxygen-mediated diseases as a pharmacological agent." @default.
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• W2079056064 date "2009-06-25" @default.
• W2079056064 modified "2023-10-16" @default.
• W2079056064 title "Preparative isolation and purification of antioxidative stilbene oligomers from <i>Vitis chunganeniss</i> using high-speed counter-current chromatography in stepwise elution mode" @default.
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• W2079056064 doi "https://doi.org/10.1002/jssc.200900033" @default.
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