FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2079064591> ?p ?o ?g. }

• W2079064591 endingPage "365" @default.
• W2079064591 startingPage "357" @default.
• W2079064591 abstract "Severe immunogenic and other debilitating human disorders potentially induced by protein aggregates have brought this phenomenon into the focus of biopharmaceutical science over the past decade. Depending on its driving forces, the process induced in the model protein rHuG-CSF may be either reversible or irreversible, resulting in the assembly of self-associated protein species or irreversible aggregates of various final morphologies. The aim of our work was to investigate the correlation between irreversible and reversible aggregation and the protective effect of non-specific formulation stabilisers, selected from the group of carbohydrates and polyols including trehalose, xylitol, cellobiitol, turanose, cellobiose, leucrose, lactitol, lyxose, and sorbitol, against both irreversible protein aggregation and reversible self-association processes of the rHuG-CSF. The formation of irreversible aggregates was thermally induced and evaluated using differential scanning calorimetry and size-exclusion chromatography. As opposed to the irreversible aggregation process, the process of self-association was induced by the agitation experiment by directly augmenting the protein solution contact surfaces. Absence of statistical connectivity between different stabilisers’ ability to inhibit self-association or aggregation reactions indicates that these are two distinct physicochemical processes with different formulation stabilizing outcomes. Reaction mechanism of thermally induced aggregation observed in the study was in line with published literature data, while the reaction mechanism for self-association process was postulated. The postulate has been verified experimentally by isothermal calorimetry and agitation set of experiments conducted after size-exclusion chromatography and asymmetrical flow field-flow fractionation separation of monomeric, dimeric, trimeric, oligomeric, and large self-associated forms detected on multi-angle light scattering, fluorescence, UV, and refractive index detectors. Besides defining the mechanism and kinetic of self-association in stabilized rHuG-CSF formulations, special attention was also paid to the shifts and ranks of the free energy of the aggregation or self-association transition states." @default.
• W2079064591 created "2016-06-24" @default.
• W2079064591 creator A5005126835 @default.
• W2079064591 creator A5021748577 @default.
• W2079064591 creator A5047175827 @default.
• W2079064591 creator A5052212095 @default.
• W2079064591 creator A5053319660 @default.
• W2079064591 creator A5063969240 @default.
• W2079064591 creator A5067485606 @default.
• W2079064591 creator A5079142893 @default.
• W2079064591 creator A5083459352 @default.
• W2079064591 creator A5086201319 @default.
• W2079064591 date "2010-11-01" @default.
• W2079064591 modified "2023-10-18" @default.
• W2079064591 title "Differences between reversible (self-association) and irreversible aggregation of rHuG-CSF in carbohydrate and polyol formulations" @default.
• W2079064591 cites W112065659 @default.
• W2079064591 cites W1454256324 @default.
• W2079064591 cites W1581858536 @default.
• W2079064591 cites W1597806993 @default.
• W2079064591 cites W1781244195 @default.
• W2079064591 cites W1964740702 @default.
• W2079064591 cites W1978709728 @default.
• W2079064591 cites W1979780600 @default.
• W2079064591 cites W1995868278 @default.
• W2079064591 cites W1997617131 @default.
• W2079064591 cites W1998140022 @default.
• W2079064591 cites W2002559713 @default.
• W2079064591 cites W2015060415 @default.
• W2079064591 cites W2028880837 @default.
• W2079064591 cites W2030450209 @default.
• W2079064591 cites W2031758006 @default.
• W2079064591 cites W2040875464 @default.
• W2079064591 cites W2047565601 @default.
• W2079064591 cites W2057598796 @default.
• W2079064591 cites W2060898668 @default.
• W2079064591 cites W2069848747 @default.
• W2079064591 cites W2072272170 @default.
• W2079064591 cites W2073443024 @default.
• W2079064591 cites W2074893134 @default.
• W2079064591 cites W2081640562 @default.
• W2079064591 cites W2086569372 @default.
• W2079064591 cites W2096780302 @default.
• W2079064591 cites W2125016536 @default.
• W2079064591 cites W2159840467 @default.
• W2079064591 doi "https://doi.org/10.1016/j.ejpb.2010.09.006" @default.
• W2079064591 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20854908" @default.
• W2079064591 hasPublicationYear "2010" @default.
• W2079064591 type Work @default.
• W2079064591 sameAs 2079064591 @default.
• W2079064591 citedByCount "12" @default.
• W2079064591 countsByYear W20790645912012 @default.
• W2079064591 countsByYear W20790645912016 @default.
• W2079064591 countsByYear W20790645912017 @default.
• W2079064591 countsByYear W20790645912018 @default.
• W2079064591 countsByYear W20790645912019 @default.
• W2079064591 countsByYear W20790645912022 @default.
• W2079064591 crossrefType "journal-article" @default.
• W2079064591 hasAuthorship W2079064591A5005126835 @default.
• W2079064591 hasAuthorship W2079064591A5021748577 @default.
• W2079064591 hasAuthorship W2079064591A5047175827 @default.
• W2079064591 hasAuthorship W2079064591A5052212095 @default.
• W2079064591 hasAuthorship W2079064591A5053319660 @default.
• W2079064591 hasAuthorship W2079064591A5063969240 @default.
• W2079064591 hasAuthorship W2079064591A5067485606 @default.
• W2079064591 hasAuthorship W2079064591A5079142893 @default.
• W2079064591 hasAuthorship W2079064591A5083459352 @default.
• W2079064591 hasAuthorship W2079064591A5086201319 @default.
• W2079064591 hasConcept C121332964 @default.
• W2079064591 hasConcept C12554922 @default.
• W2079064591 hasConcept C136238340 @default.
• W2079064591 hasConcept C156911925 @default.
• W2079064591 hasConcept C166940927 @default.
• W2079064591 hasConcept C178790620 @default.
• W2079064591 hasConcept C181199279 @default.
• W2079064591 hasConcept C185592680 @default.
• W2079064591 hasConcept C193363816 @default.
• W2079064591 hasConcept C2776779347 @default.
• W2079064591 hasConcept C2777239854 @default.
• W2079064591 hasConcept C2778452849 @default.
• W2079064591 hasConcept C39519442 @default.
• W2079064591 hasConcept C43617362 @default.
• W2079064591 hasConcept C521977710 @default.
• W2079064591 hasConcept C55493867 @default.
• W2079064591 hasConcept C86803240 @default.
• W2079064591 hasConcept C97355855 @default.
• W2079064591 hasConceptScore W2079064591C121332964 @default.
• W2079064591 hasConceptScore W2079064591C12554922 @default.
• W2079064591 hasConceptScore W2079064591C136238340 @default.
• W2079064591 hasConceptScore W2079064591C156911925 @default.
• W2079064591 hasConceptScore W2079064591C166940927 @default.
• W2079064591 hasConceptScore W2079064591C178790620 @default.
• W2079064591 hasConceptScore W2079064591C181199279 @default.
• W2079064591 hasConceptScore W2079064591C185592680 @default.
• W2079064591 hasConceptScore W2079064591C193363816 @default.
• W2079064591 hasConceptScore W2079064591C2776779347 @default.
• W2079064591 hasConceptScore W2079064591C2777239854 @default.
• W2079064591 hasConceptScore W2079064591C2778452849 @default.
• W2079064591 hasConceptScore W2079064591C39519442 @default.

• next