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• W2079072755 startingPage "1705" @default.
• W2079072755 abstract "Proteolysis constitutes a major post-translational modification but specificity and substrate selectivity of numerous proteases have remained elusive. In this review, we highlight how advanced techniques in the areas of proteomics and activity-based probes can be used to investigate i) protease active site specificity; ii) protease in vivo substrates; iii) protease contribution to proteome homeostasis and composition; and iv) detection and localization of active proteases. Peptide libraries together with genetical or biochemical selection have traditionally been used for active site profiling of proteases. These are now complemented by proteome-derived peptide libraries that simultaneously determine prime and non-prime specificity and characterize subsite cooperativity. Cell-contextual discovery of protease substrates is rendered possible by techniques that isolate and quantitate protein termini. Here, a novel approach termed Terminal Amine Isotopic Labeling of Substrates (TAILS) provides an integrated platform for substrate discovery and appropriate statistical evaluation of terminal peptide identification and quantification. Proteolytically generated carboxy-termini can now also be analyzed on a proteome-wide level. Proteolytic regulation of proteome composition is monitored by quantitative proteomic approaches employing stable isotope coding or label free quantification. Activity-based probes specifically recognize active proteases. In proteomic screens, they can be used to detect and quantitate proteolytic activity while their application in cellular histology allows to locate proteolytic activity in situ. Activity-based probes – especially in conjunction with positron emission tomography – are also promising tools to monitor proteolytic activities on an organism-wide basis with a focus on in vivo tumor imaging. Together, this array of methodological possibilities enables unveiling physiological protease substrate repertoires and defining protease function in the cellular- and organism-wide context." @default.
• W2079072755 created "2016-06-24" @default.
• W2079072755 creator A5050843400 @default.
• W2079072755 creator A5076623682 @default.
• W2079072755 date "2010-11-01" @default.
• W2079072755 modified "2023-09-29" @default.
• W2079072755 title "Proteomic techniques and activity-based probes for the system-wide study of proteolysis" @default.
• W2079072755 cites W1555862556 @default.
• W2079072755 cites W1572736799 @default.
• W2079072755 cites W1607581180 @default.
• W2079072755 cites W1966581230 @default.
• W2079072755 cites W1970953442 @default.
• W2079072755 cites W1971199972 @default.
• W2079072755 cites W1973019272 @default.
• W2079072755 cites W1975558168 @default.
• W2079072755 cites W1977660972 @default.
• W2079072755 cites W1982516441 @default.
• W2079072755 cites W1984483611 @default.
• W2079072755 cites W1995896536 @default.
• W2079072755 cites W1997719075 @default.
• W2079072755 cites W1997871576 @default.
• W2079072755 cites W1998645626 @default.
• W2079072755 cites W1999376552 @default.
• W2079072755 cites W1999891600 @default.
• W2079072755 cites W2000831618 @default.
• W2079072755 cites W2000919336 @default.
• W2079072755 cites W2004473457 @default.
• W2079072755 cites W2004496049 @default.
• W2079072755 cites W2004588605 @default.
• W2079072755 cites W2006786242 @default.
• W2079072755 cites W2009154911 @default.
• W2079072755 cites W2009904294 @default.
• W2079072755 cites W2012420955 @default.
• W2079072755 cites W2013102869 @default.
• W2079072755 cites W2013710989 @default.
• W2079072755 cites W2017867429 @default.
• W2079072755 cites W2019427729 @default.
• W2079072755 cites W2022045852 @default.
• W2079072755 cites W2022812138 @default.
• W2079072755 cites W2025554716 @default.
• W2079072755 cites W2028097805 @default.
• W2079072755 cites W2029901102 @default.
• W2079072755 cites W2030332870 @default.
• W2079072755 cites W2035590596 @default.
• W2079072755 cites W2042907121 @default.
• W2079072755 cites W2043073885 @default.
• W2079072755 cites W2044621914 @default.
• W2079072755 cites W2045528982 @default.
• W2079072755 cites W2046974910 @default.
• W2079072755 cites W2049619834 @default.
• W2079072755 cites W2050358188 @default.
• W2079072755 cites W2050756511 @default.
• W2079072755 cites W2051807429 @default.
• W2079072755 cites W2060470491 @default.
• W2079072755 cites W2060688195 @default.
• W2079072755 cites W2060712542 @default.
• W2079072755 cites W2061945526 @default.
• W2079072755 cites W2062919894 @default.
• W2079072755 cites W2065489689 @default.
• W2079072755 cites W2065518020 @default.
• W2079072755 cites W2068819766 @default.
• W2079072755 cites W2069508712 @default.
• W2079072755 cites W2070852203 @default.
• W2079072755 cites W2074912245 @default.
• W2079072755 cites W2075503331 @default.
• W2079072755 cites W2079888227 @default.
• W2079072755 cites W2080198169 @default.
• W2079072755 cites W2081282825 @default.
• W2079072755 cites W2083015173 @default.
• W2079072755 cites W2084603756 @default.
• W2079072755 cites W2086554981 @default.
• W2079072755 cites W2088569816 @default.
• W2079072755 cites W2090408997 @default.
• W2079072755 cites W2090499262 @default.
• W2079072755 cites W2091273971 @default.
• W2079072755 cites W2094932777 @default.
• W2079072755 cites W2096264619 @default.
• W2079072755 cites W2097033861 @default.
• W2079072755 cites W2097619603 @default.
• W2079072755 cites W2107806009 @default.
• W2079072755 cites W2110691960 @default.
• W2079072755 cites W2111208057 @default.
• W2079072755 cites W2112395233 @default.
• W2079072755 cites W2113219548 @default.
• W2079072755 cites W2114322774 @default.
• W2079072755 cites W2119294728 @default.
• W2079072755 cites W2120016245 @default.
• W2079072755 cites W2126133541 @default.
• W2079072755 cites W2136255360 @default.
• W2079072755 cites W2136588287 @default.
• W2079072755 cites W2137043026 @default.
• W2079072755 cites W2137579259 @default.
• W2079072755 cites W2140946052 @default.
• W2079072755 cites W2146267741 @default.
• W2079072755 cites W2147105537 @default.
• W2079072755 cites W2147292812 @default.
• W2079072755 cites W2148528976 @default.
• W2079072755 cites W2153205700 @default.

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