Matches in SemOpenAlex for { <https://semopenalex.org/work/W2079077279> ?p ?o ?g. }
- W2079077279 endingPage "688" @default.
- W2079077279 startingPage "685" @default.
- W2079077279 abstract "Kramers developed the theory on how chemical reaction rates are influenced by the viscosity of the medium. At the viscosity of water, the kinetics of unimolecular reactions are described by diffusion of a Brownian particle over a free-energy barrier separating reactants and products. For reactions in solution this famous theory extended Eyring's transition state theory, and is widely applied in physics, chemistry and biology, including to reactions as complex as protein folding. Because the diffusion coefficient of Kramers' theory is determined by the dynamics in the sparsely populated region of the barrier top, its properties have not been directly measured for any molecular system. Here we show that the Kramers diffusion coefficient and free-energy barrier can be characterized by measuring the temperature- and viscosity-dependence of the transition path time for protein folding. The transition path is the small fraction of an equilibrium trajectory for a single molecule when the free-energy barrier separating two states is actually crossed. Its duration, the transition path time, can now be determined from photon trajectories for single protein molecules undergoing folding/unfolding transitions. Our finding of a long transition path time with an unusually small solvent viscosity dependence suggests that internal friction as well as solvent friction determine the Kramers diffusion coefficient for α-helical proteins, as opposed to a breakdown of his theory, which occurs for many small-molecule reactions. It is noteworthy that the new and fundamental information concerning Kramers' theory and the dynamics of barrier crossings obtained here come from experiments on a protein rather than a much simpler chemical or physical system." @default.
- W2079077279 created "2016-06-24" @default.
- W2079077279 creator A5075318212 @default.
- W2079077279 creator A5083376970 @default.
- W2079077279 date "2013-10-01" @default.
- W2079077279 modified "2023-10-10" @default.
- W2079077279 title "Single-molecule fluorescence probes dynamics of barrier crossing" @default.
- W2079077279 cites W1966103056 @default.
- W2079077279 cites W1972675000 @default.
- W2079077279 cites W1981716245 @default.
- W2079077279 cites W1987362936 @default.
- W2079077279 cites W1991133427 @default.
- W2079077279 cites W1998045383 @default.
- W2079077279 cites W2013536460 @default.
- W2079077279 cites W2013906676 @default.
- W2079077279 cites W2016788463 @default.
- W2079077279 cites W2017975247 @default.
- W2079077279 cites W2024423406 @default.
- W2079077279 cites W2024982003 @default.
- W2079077279 cites W2029137764 @default.
- W2079077279 cites W2031243389 @default.
- W2079077279 cites W2038701810 @default.
- W2079077279 cites W2042088844 @default.
- W2079077279 cites W2063392510 @default.
- W2079077279 cites W2067236515 @default.
- W2079077279 cites W2071075252 @default.
- W2079077279 cites W2081926098 @default.
- W2079077279 cites W2083006981 @default.
- W2079077279 cites W2085266415 @default.
- W2079077279 cites W2085809095 @default.
- W2079077279 cites W2091983181 @default.
- W2079077279 cites W2092144432 @default.
- W2079077279 cites W2100483890 @default.
- W2079077279 cites W2102074396 @default.
- W2079077279 cites W2114027916 @default.
- W2079077279 cites W2125838338 @default.
- W2079077279 cites W2140213897 @default.
- W2079077279 cites W2145326890 @default.
- W2079077279 cites W2148723336 @default.
- W2079077279 cites W2150614072 @default.
- W2079077279 cites W2157792819 @default.
- W2079077279 cites W2165414288 @default.
- W2079077279 cites W2314276379 @default.
- W2079077279 cites W2951712345 @default.
- W2079077279 cites W3104305066 @default.
- W2079077279 doi "https://doi.org/10.1038/nature12649" @default.
- W2079077279 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4009947" @default.
- W2079077279 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24153185" @default.
- W2079077279 hasPublicationYear "2013" @default.
- W2079077279 type Work @default.
- W2079077279 sameAs 2079077279 @default.
- W2079077279 citedByCount "188" @default.
- W2079077279 countsByYear W20790772792013 @default.
- W2079077279 countsByYear W20790772792014 @default.
- W2079077279 countsByYear W20790772792015 @default.
- W2079077279 countsByYear W20790772792016 @default.
- W2079077279 countsByYear W20790772792017 @default.
- W2079077279 countsByYear W20790772792018 @default.
- W2079077279 countsByYear W20790772792019 @default.
- W2079077279 countsByYear W20790772792020 @default.
- W2079077279 countsByYear W20790772792021 @default.
- W2079077279 countsByYear W20790772792022 @default.
- W2079077279 countsByYear W20790772792023 @default.
- W2079077279 crossrefType "journal-article" @default.
- W2079077279 hasAuthorship W2079077279A5075318212 @default.
- W2079077279 hasAuthorship W2079077279A5083376970 @default.
- W2079077279 hasBestOaLocation W20790772792 @default.
- W2079077279 hasConcept C112401455 @default.
- W2079077279 hasConcept C119599485 @default.
- W2079077279 hasConcept C119621388 @default.
- W2079077279 hasConcept C121332964 @default.
- W2079077279 hasConcept C127172972 @default.
- W2079077279 hasConcept C127413603 @default.
- W2079077279 hasConcept C130603088 @default.
- W2079077279 hasConcept C148898269 @default.
- W2079077279 hasConcept C159467904 @default.
- W2079077279 hasConcept C161790260 @default.
- W2079077279 hasConcept C178790620 @default.
- W2079077279 hasConcept C185592680 @default.
- W2079077279 hasConcept C204328495 @default.
- W2079077279 hasConcept C2776545253 @default.
- W2079077279 hasConcept C32909587 @default.
- W2079077279 hasConcept C55493867 @default.
- W2079077279 hasConcept C57476982 @default.
- W2079077279 hasConcept C62520636 @default.
- W2079077279 hasConcept C69357855 @default.
- W2079077279 hasConcept C74650414 @default.
- W2079077279 hasConcept C89031862 @default.
- W2079077279 hasConcept C93391505 @default.
- W2079077279 hasConcept C97355855 @default.
- W2079077279 hasConceptScore W2079077279C112401455 @default.
- W2079077279 hasConceptScore W2079077279C119599485 @default.
- W2079077279 hasConceptScore W2079077279C119621388 @default.
- W2079077279 hasConceptScore W2079077279C121332964 @default.
- W2079077279 hasConceptScore W2079077279C127172972 @default.
- W2079077279 hasConceptScore W2079077279C127413603 @default.
- W2079077279 hasConceptScore W2079077279C130603088 @default.
- W2079077279 hasConceptScore W2079077279C148898269 @default.