FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2079078732> ?p ?o ?g. }

• W2079078732 endingPage "4945" @default.
• W2079078732 startingPage "4935" @default.
• W2079078732 abstract "After induction of DNA double-strand breaks (DSB) two repair systems, the error-prone `nonhomologous end joining' (NHEJ) and the more accurate `homologous recombination repair' (HRR) can compete for the same individual DSB site. In the human keratinocyte cell line, HaCaT, we have tested the spatial co-localisation and the temporal sequence of events. We used UV-A (365 nm) as a damaging agent, which can be applied in clearly defined doses and can lead to rare DSBs via propagation of clustered single-strand breaks (SSBs). DNA fragmentation and repair was measured by the Comet assay and persisting DSBs were quantified by the micronucleus assay. Direct DSB detection was performed by immunohistochemical labelling of γ-H2AX, a phosphorylated histone that is assumed to form one foci per DSB. Intra- and inter-pathway interactions were quantified by co-localisation, FRET imaging and by co-immunoprecipitation (Co-IP) of XRCC4, DNA-PK and Ku70 as representatives of NHEJ, Rad51 and Rad52 for HRR and γ-H2AX, Mre11 and Rad50 as representatives of both pathways. In G2 cells, where both systems are available, the temporal sequence after irradiation is: (1) γ-H2AX (2) Mre11 (3) DNA-PK Rad51 (4) XRCC4. That is, the first two proteins involved in both pathways `label' the damaged site and initiate repair, followed by the NHEJ, which is temporally overlapping with HRR activity. Taking all these observations together we suggest that a cell tries to repair DSBs with a combination of both HRR and NHEJ, if available." @default.
• W2079078732 created "2016-06-24" @default.
• W2079078732 creator A5003366735 @default.
• W2079078732 creator A5045761618 @default.
• W2079078732 date "2004-10-01" @default.
• W2079078732 modified "2023-10-16" @default.
• W2079078732 title "After double-strand break induction by UV-A, homologous recombination and nonhomologous end joining cooperate at the same DSB if both systems are available" @default.
• W2079078732 cites W1909122431 @default.
• W2079078732 cites W1917565160 @default.
• W2079078732 cites W1981085859 @default.
• W2079078732 cites W1985775217 @default.
• W2079078732 cites W1987345634 @default.
• W2079078732 cites W1992632955 @default.
• W2079078732 cites W2019219567 @default.
• W2079078732 cites W2019405732 @default.
• W2079078732 cites W2022972203 @default.
• W2079078732 cites W2026711486 @default.
• W2079078732 cites W2034205457 @default.
• W2079078732 cites W2040235808 @default.
• W2079078732 cites W2040904778 @default.
• W2079078732 cites W2052921761 @default.
• W2079078732 cites W2061737909 @default.
• W2079078732 cites W2067620147 @default.
• W2079078732 cites W2068781703 @default.
• W2079078732 cites W2070636723 @default.
• W2079078732 cites W2072373620 @default.
• W2079078732 cites W2073602724 @default.
• W2079078732 cites W2080357756 @default.
• W2079078732 cites W2085124053 @default.
• W2079078732 cites W2089138444 @default.
• W2079078732 cites W2092629964 @default.
• W2079078732 cites W2096136751 @default.
• W2079078732 cites W2099627146 @default.
• W2079078732 cites W2102557954 @default.
• W2079078732 cites W2126902508 @default.
• W2079078732 cites W2130883309 @default.
• W2079078732 cites W2133373186 @default.
• W2079078732 cites W2143464311 @default.
• W2079078732 cites W2155886452 @default.
• W2079078732 cites W2160532161 @default.
• W2079078732 cites W2163216399 @default.
• W2079078732 cites W2163367614 @default.
• W2079078732 cites W2169637630 @default.
• W2079078732 cites W2171584212 @default.
• W2079078732 cites W2174653327 @default.
• W2079078732 cites W2175536586 @default.
• W2079078732 doi "https://doi.org/10.1242/jcs.01355" @default.
• W2079078732 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15367581" @default.
• W2079078732 hasPublicationYear "2004" @default.
• W2079078732 type Work @default.
• W2079078732 sameAs 2079078732 @default.
• W2079078732 citedByCount "102" @default.
• W2079078732 countsByYear W20790787322012 @default.
• W2079078732 countsByYear W20790787322013 @default.
• W2079078732 countsByYear W20790787322014 @default.
• W2079078732 countsByYear W20790787322015 @default.
• W2079078732 countsByYear W20790787322016 @default.
• W2079078732 countsByYear W20790787322017 @default.
• W2079078732 countsByYear W20790787322018 @default.
• W2079078732 countsByYear W20790787322019 @default.
• W2079078732 countsByYear W20790787322020 @default.
• W2079078732 countsByYear W20790787322021 @default.
• W2079078732 countsByYear W20790787322022 @default.
• W2079078732 countsByYear W20790787322023 @default.
• W2079078732 crossrefType "journal-article" @default.
• W2079078732 hasAuthorship W2079078732A5003366735 @default.
• W2079078732 hasAuthorship W2079078732A5045761618 @default.
• W2079078732 hasBestOaLocation W20790787321 @default.
• W2079078732 hasConcept C102744134 @default.
• W2079078732 hasConcept C104317684 @default.
• W2079078732 hasConcept C104451858 @default.
• W2079078732 hasConcept C118995209 @default.
• W2079078732 hasConcept C122917832 @default.
• W2079078732 hasConcept C134935766 @default.
• W2079078732 hasConcept C143425029 @default.
• W2079078732 hasConcept C145803527 @default.
• W2079078732 hasConcept C153911025 @default.
• W2079078732 hasConcept C193104389 @default.
• W2079078732 hasConcept C2779012473 @default.
• W2079078732 hasConcept C2780919815 @default.
• W2079078732 hasConcept C54355233 @default.
• W2079078732 hasConcept C552990157 @default.
• W2079078732 hasConcept C64927066 @default.
• W2079078732 hasConcept C81885089 @default.
• W2079078732 hasConcept C86339819 @default.
• W2079078732 hasConcept C86803240 @default.
• W2079078732 hasConcept C94966510 @default.
• W2079078732 hasConcept C95444343 @default.
• W2079078732 hasConceptScore W2079078732C102744134 @default.
• W2079078732 hasConceptScore W2079078732C104317684 @default.
• W2079078732 hasConceptScore W2079078732C104451858 @default.
• W2079078732 hasConceptScore W2079078732C118995209 @default.
• W2079078732 hasConceptScore W2079078732C122917832 @default.
• W2079078732 hasConceptScore W2079078732C134935766 @default.
• W2079078732 hasConceptScore W2079078732C143425029 @default.
• W2079078732 hasConceptScore W2079078732C145803527 @default.
• W2079078732 hasConceptScore W2079078732C153911025 @default.
• W2079078732 hasConceptScore W2079078732C193104389 @default.

• next