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• W2079085498 abstract "The formation and decomposition (postsource decay, PSD) of anionic adducts in negative ion matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometry have been studied. Chloride, a small inorganic anion, has been found to form stable anionic adducts with a variety of neutral oligosaccharides that can survive the MALDI process to give readily identifiable signals (with characteristic isotope patterns) allowing subpicomole detection in the best cases. The matrixes that can aid the formation of chloride adducts of oligosaccharides have gas-phase acidities lower than or close to that of HCl (1373 kJ/mol). In PSD experiments, precursor chloride adducts of oligosaccharides yield fragment ions that retain the charge on the sugar molecule rather than solely forming Cl-, and these fragments can provide structurally informative product ions. In negative ion MALDI, highly acidic oligosaccharides do not form adducts with chloride anions, but mildly acidic saccharides (e.g., containing a carboxylic acid group) form both deprotonated molecules and chloride adducts, and each may provide structural information concerning the oligosaccharide upon decomposition." @default.
• W2079085498 created "2016-06-24" @default.
• W2079085498 creator A5018244995 @default.
• W2079085498 creator A5038900532 @default.
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• W2079085498 date "2003-03-01" @default.
• W2079085498 modified "2023-09-29" @default.
• W2079085498 title "Anionic Adducts of Oligosaccharides by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry" @default.
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• W2079085498 doi "https://doi.org/10.1021/ac0205513" @default.
• W2079085498 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12705596" @default.
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