Matches in SemOpenAlex for { <https://semopenalex.org/work/W2079086920> ?p ?o ?g. }
- W2079086920 endingPage "529" @default.
- W2079086920 startingPage "515" @default.
- W2079086920 abstract "We previously showed that plasmids containing a mammalian replication initiation region and a matrix attachment region were efficiently amplified to few thousand copies per cell, and that they formed extrachromosomal double minutes (DMs) or chromosomal homogeneously staining regions (HSRs). In these structures, the plasmid sequence was arranged as a tandem repeats, and we suggested a mechanism of plasmid amplification. Since amplification was very efficient, easy, and convenient, it might be adapted to a novel method for protein production. In the current study, we found that gene expression from the tandem plasmid repeat was suppressed. We identified several strategies to overcome this suppression, including: (1) use of higher concentrations of antibiotic during cell selection; (2) treatment of cells with agents that influence DNA methylation (5-azacytidine) or histone acetylation (butyrate); (3) co-amplification of an insulator sequence; and (4) co-amplification of sequences that encode a transcriptional activator. Expression from the plasmid repeat was always higher at DMs compared to HSRs. We found that continuous activation of a plasmid-encoded inducible promoter prevented the generation of long HSRs, and favored amplification at DMs. Consistent with this finding, there was a synergistic effect of transcriptional activation and inhibition of DNA methylation on the fragmentation of long HSRs and the generation of DMs and short HSRs. Our results indicate that both transcriptional activation and DNA methylation regulate the interconversion between extra- and intra-chromosomal gene amplification. These results have important implications for both protein production technology, and the generation of chromosomal abnormalities found in human cancer cells. J. Cell. Biochem. 102: 515–529, 2007. © 2007 Wiley-Liss, Inc." @default.
- W2079086920 created "2016-06-24" @default.
- W2079086920 creator A5020512644 @default.
- W2079086920 creator A5047485957 @default.
- W2079086920 creator A5056136585 @default.
- W2079086920 creator A5077109718 @default.
- W2079086920 date "2007-01-01" @default.
- W2079086920 modified "2023-09-27" @default.
- W2079086920 title "Interconversion of intra- and extra-chromosomal sites of gene amplification by modulation of gene expression and DNA methylation" @default.
- W2079086920 cites W1972199640 @default.
- W2079086920 cites W1976365653 @default.
- W2079086920 cites W1976543436 @default.
- W2079086920 cites W1978716989 @default.
- W2079086920 cites W1996679276 @default.
- W2079086920 cites W2005029480 @default.
- W2079086920 cites W2007811800 @default.
- W2079086920 cites W2011480379 @default.
- W2079086920 cites W2013876995 @default.
- W2079086920 cites W2013892708 @default.
- W2079086920 cites W2014401442 @default.
- W2079086920 cites W2022826887 @default.
- W2079086920 cites W2048481294 @default.
- W2079086920 cites W2052541734 @default.
- W2079086920 cites W2059585949 @default.
- W2079086920 cites W2061829784 @default.
- W2079086920 cites W2062083469 @default.
- W2079086920 cites W2065189089 @default.
- W2079086920 cites W2077398494 @default.
- W2079086920 cites W2080976322 @default.
- W2079086920 cites W2081949650 @default.
- W2079086920 cites W2089033365 @default.
- W2079086920 cites W2092956510 @default.
- W2079086920 cites W2095880532 @default.
- W2079086920 cites W2097309207 @default.
- W2079086920 cites W2105190923 @default.
- W2079086920 cites W2105427468 @default.
- W2079086920 cites W2115281458 @default.
- W2079086920 cites W2118540273 @default.
- W2079086920 cites W2120321636 @default.
- W2079086920 cites W2122252026 @default.
- W2079086920 cites W2124181908 @default.
- W2079086920 cites W2139509558 @default.
- W2079086920 cites W2140418170 @default.
- W2079086920 cites W2142864992 @default.
- W2079086920 cites W2164976222 @default.
- W2079086920 cites W2189013505 @default.
- W2079086920 cites W2329147600 @default.
- W2079086920 doi "https://doi.org/10.1002/jcb.21313" @default.
- W2079086920 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17390337" @default.
- W2079086920 hasPublicationYear "2007" @default.
- W2079086920 type Work @default.
- W2079086920 sameAs 2079086920 @default.
- W2079086920 citedByCount "21" @default.
- W2079086920 countsByYear W20790869202012 @default.
- W2079086920 countsByYear W20790869202013 @default.
- W2079086920 countsByYear W20790869202016 @default.
- W2079086920 countsByYear W20790869202017 @default.
- W2079086920 countsByYear W20790869202019 @default.
- W2079086920 countsByYear W20790869202020 @default.
- W2079086920 countsByYear W20790869202021 @default.
- W2079086920 countsByYear W20790869202023 @default.
- W2079086920 crossrefType "journal-article" @default.
- W2079086920 hasAuthorship W2079086920A5020512644 @default.
- W2079086920 hasAuthorship W2079086920A5047485957 @default.
- W2079086920 hasAuthorship W2079086920A5056136585 @default.
- W2079086920 hasAuthorship W2079086920A5077109718 @default.
- W2079086920 hasConcept C104317684 @default.
- W2079086920 hasConcept C150194340 @default.
- W2079086920 hasConcept C153911025 @default.
- W2079086920 hasConcept C190727270 @default.
- W2079086920 hasConcept C22744801 @default.
- W2079086920 hasConcept C54355233 @default.
- W2079086920 hasConcept C552990157 @default.
- W2079086920 hasConcept C64927066 @default.
- W2079086920 hasConcept C7602840 @default.
- W2079086920 hasConcept C86803240 @default.
- W2079086920 hasConcept C89684784 @default.
- W2079086920 hasConceptScore W2079086920C104317684 @default.
- W2079086920 hasConceptScore W2079086920C150194340 @default.
- W2079086920 hasConceptScore W2079086920C153911025 @default.
- W2079086920 hasConceptScore W2079086920C190727270 @default.
- W2079086920 hasConceptScore W2079086920C22744801 @default.
- W2079086920 hasConceptScore W2079086920C54355233 @default.
- W2079086920 hasConceptScore W2079086920C552990157 @default.
- W2079086920 hasConceptScore W2079086920C64927066 @default.
- W2079086920 hasConceptScore W2079086920C7602840 @default.
- W2079086920 hasConceptScore W2079086920C86803240 @default.
- W2079086920 hasConceptScore W2079086920C89684784 @default.
- W2079086920 hasIssue "2" @default.
- W2079086920 hasLocation W20790869201 @default.
- W2079086920 hasLocation W20790869202 @default.
- W2079086920 hasOpenAccess W2079086920 @default.
- W2079086920 hasPrimaryLocation W20790869201 @default.
- W2079086920 hasRelatedWork W1571771606 @default.
- W2079086920 hasRelatedWork W2009966535 @default.
- W2079086920 hasRelatedWork W2043199743 @default.
- W2079086920 hasRelatedWork W2076047342 @default.
- W2079086920 hasRelatedWork W2085507831 @default.