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- W2079088748 abstract "Abstract Bovine spermatozoa cryopreserved in media containing two combinations of antibiotics were analyzed using a FACS analyzer and an EPICS V flow cytometer. Duplicate samples from 10 bulls with information on nonreturn rates were examined. Semen was processed using either penicillin-streptomycin-polymyxin-lincospectin (Regimen I) or gentamicin-tylosin-lincospectin (Regimen II). Spermatozoa were thawed at 37°C and incubated for 0, 1.5, and 3.0 h in 10 mM HEPES buffer at 39°C. Aliquots were then stained using a combination of 5(and-6)-carboxy-4′,5′ dimethyl fluorescein diacetate and hydroethidine. Flow cytometric analysis of the stained samples identified five fluorescent populations for the FACS analyzer and three for the EPICS V. There were no significant differences in the number of spermatozoa within similar fluorescent populations between antibiotic Regimens I and II for all incubation times with one exception. One spermatozoal population (FACS Analyzer), after 1.5 h of incubation, contained more cells with decreased viability (P" @default.
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- W2079088748 date "1990-06-01" @default.
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- W2079088748 title "Flow cytometric evaluation of cryopreserved bovine spermatozoa processed using a new antibiotic combination" @default.
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- W2079088748 doi "https://doi.org/10.1016/0093-691x(90)90039-v" @default.
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