Matches in SemOpenAlex for { <https://semopenalex.org/work/W2079131909> ?p ?o ?g. }
- W2079131909 endingPage "2389" @default.
- W2079131909 startingPage "2367" @default.
- W2079131909 abstract "Protein (de)phosphorylation plays an important role in plants. To provide a robust foundation for subcellular phosphorylation signaling network analysis and kinase-substrate relationships, we performed a meta-analysis of 27 published and unpublished in-house mass spectrometry-based phospho-proteome data sets for Arabidopsis thaliana covering a range of processes, (non)photosynthetic tissue types, and cell cultures. This resulted in an assembly of 60,366 phospho-peptides matching to 8141 nonredundant proteins. Filtering the data for quality and consistency generated a set of medium and a set of high confidence phospho-proteins and their assigned phospho-sites. The relation between single and multiphosphorylated peptides is discussed. The distribution of p-proteins across cellular functions and subcellular compartments was determined and showed overrepresentation of protein kinases. Extensive differences in frequency of pY were found between individual studies due to proteomics and mass spectrometry workflows. Interestingly, pY was underrepresented in peroxisomes but overrepresented in mitochondria. Using motif-finding algorithms motif-x and MMFPh at high stringency, we identified compartmentalization of phosphorylation motifs likely reflecting localized kinase activity. The filtering of the data assembly improved signal/noise ratio for such motifs. Identified motifs were linked to kinases through (bioinformatic) enrichment analysis. This study also provides insight into the challenges/pitfalls of using large-scale phospho-proteomic data sets to nonexperts." @default.
- W2079131909 created "2016-06-24" @default.
- W2079131909 creator A5009637778 @default.
- W2079131909 creator A5046326063 @default.
- W2079131909 creator A5061247186 @default.
- W2079131909 creator A5090816444 @default.
- W2079131909 date "2014-06-01" @default.
- W2079131909 modified "2023-10-14" @default.
- W2079131909 title "Meta-Analysis of <i>Arabidopsis thaliana</i> Phospho-Proteomics Data Reveals Compartmentalization of Phosphorylation Motifs" @default.
- W2079131909 cites W1509772503 @default.
- W2079131909 cites W1539343861 @default.
- W2079131909 cites W1547329255 @default.
- W2079131909 cites W1571090306 @default.
- W2079131909 cites W1917156407 @default.
- W2079131909 cites W1964846007 @default.
- W2079131909 cites W1970544448 @default.
- W2079131909 cites W1972172786 @default.
- W2079131909 cites W1973673322 @default.
- W2079131909 cites W1980506273 @default.
- W2079131909 cites W1984804967 @default.
- W2079131909 cites W1986662234 @default.
- W2079131909 cites W1988805210 @default.
- W2079131909 cites W1992006779 @default.
- W2079131909 cites W1995666296 @default.
- W2079131909 cites W1995989434 @default.
- W2079131909 cites W1997318763 @default.
- W2079131909 cites W2000042427 @default.
- W2079131909 cites W2003642059 @default.
- W2079131909 cites W2005000195 @default.
- W2079131909 cites W2006595529 @default.
- W2079131909 cites W2007958560 @default.
- W2079131909 cites W2010140810 @default.
- W2079131909 cites W2011615884 @default.
- W2079131909 cites W2015227064 @default.
- W2079131909 cites W2018413328 @default.
- W2079131909 cites W2024178204 @default.
- W2079131909 cites W2031028006 @default.
- W2079131909 cites W2033199777 @default.
- W2079131909 cites W2034293882 @default.
- W2079131909 cites W2038477741 @default.
- W2079131909 cites W2040709819 @default.
- W2079131909 cites W2048858800 @default.
- W2079131909 cites W2049232794 @default.
- W2079131909 cites W2051147053 @default.
- W2079131909 cites W2052141890 @default.
- W2079131909 cites W2052841916 @default.
- W2079131909 cites W2055143728 @default.
- W2079131909 cites W2060747252 @default.
- W2079131909 cites W2060868346 @default.
- W2079131909 cites W2064050139 @default.
- W2079131909 cites W2064483138 @default.
- W2079131909 cites W2068244478 @default.
- W2079131909 cites W2076067064 @default.
- W2079131909 cites W2079269139 @default.
- W2079131909 cites W2079817279 @default.
- W2079131909 cites W2086385989 @default.
- W2079131909 cites W2089466813 @default.
- W2079131909 cites W2094732767 @default.
- W2079131909 cites W2094927094 @default.
- W2079131909 cites W2095787767 @default.
- W2079131909 cites W2095917175 @default.
- W2079131909 cites W2097455782 @default.
- W2079131909 cites W2098715150 @default.
- W2079131909 cites W2099682053 @default.
- W2079131909 cites W2102025585 @default.
- W2079131909 cites W2103622787 @default.
- W2079131909 cites W2104670564 @default.
- W2079131909 cites W2105597591 @default.
- W2079131909 cites W2106396116 @default.
- W2079131909 cites W2107627065 @default.
- W2079131909 cites W2114508508 @default.
- W2079131909 cites W2117912943 @default.
- W2079131909 cites W2117992912 @default.
- W2079131909 cites W2120261223 @default.
- W2079131909 cites W2122210253 @default.
- W2079131909 cites W2122664708 @default.
- W2079131909 cites W2123480261 @default.
- W2079131909 cites W2123799869 @default.
- W2079131909 cites W2128268951 @default.
- W2079131909 cites W2128946722 @default.
- W2079131909 cites W2132775653 @default.
- W2079131909 cites W2133564336 @default.
- W2079131909 cites W2135238438 @default.
- W2079131909 cites W2137617230 @default.
- W2079131909 cites W2139069348 @default.
- W2079131909 cites W2139750884 @default.
- W2079131909 cites W2140392387 @default.
- W2079131909 cites W2141357431 @default.
- W2079131909 cites W2141708714 @default.
- W2079131909 cites W2152287977 @default.
- W2079131909 cites W2153190698 @default.
- W2079131909 cites W2153777219 @default.
- W2079131909 cites W2155814768 @default.
- W2079131909 cites W2156916536 @default.
- W2079131909 cites W2158513960 @default.
- W2079131909 cites W2159644279 @default.
- W2079131909 cites W2164234055 @default.
- W2079131909 cites W2168483798 @default.