FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2079133664> ?p ?o ?g. }

• W2079133664 endingPage "1449" @default.
• W2079133664 startingPage "1439" @default.
• W2079133664 abstract "Bioengineered fiber substrates are increasingly studied as a means to promote regeneration and remodeling in the injured central nervous system (CNS). Previous reports largely focused on the ability of oriented scaffolds to bridge injured regions and direct outgrowth of axonal projections. In the present work, we explored the effects of electrospun microfibers on the migration and physiological properties of brain astroglial cells. Primary rat astrocytes were cultured on either fibronectin-coated poly-l-lactic acid (PLLA) films, fibronectin-coated randomly oriented PLLA electrospun fibers, or fibronectin-coated aligned PLLA electrospun fibers. Aligned PLLA fibers strongly altered astrocytic morphology, orienting cell processes, actin microfilaments, and microtubules along the length of the fibers. On aligned fibers, astrocytes also significantly increased their migration rates in the direction of fiber orientation. We further investigated if fiber topography modifies astrocytic neuroprotective properties, namely glutamate and glutamine transport and metabolism. This was done by quantifying changes in mRNA expression (qRT-PCR) and protein levels (Western blotting) for a battery of relevant biomolecules. Interestingly, we found that cells grown on random and/or aligned fibers increased the expression levels of two glutamate transporters, GLAST and GLT-1, and an important metabolic enzyme, glutamine synthetase, as compared to the fibronectin-coated films. Functional assays revealed increases in glutamate transport rates due to GLT-1 mediated uptake, which was largely determined by the dihydrokainate-sensitive GLT-1. Overall, this study suggests that aligned PLLA fibers can promote directed astrocytic migration, and, of most importance, our in vitro results indicate for the first time that electrospun PLLA fibers can positively modify neuroprotective properties of glial cells by increasing rates of glutamate uptake." @default.
• W2079133664 created "2016-06-24" @default.
• W2079133664 creator A5015163321 @default.
• W2079133664 creator A5024938105 @default.
• W2079133664 creator A5046498224 @default.
• W2079133664 creator A5077395570 @default.
• W2079133664 creator A5081624863 @default.
• W2079133664 creator A5085880004 @default.
• W2079133664 creator A5090293126 @default.
• W2079133664 date "2014-02-01" @default.
• W2079133664 modified "2023-10-10" @default.
• W2079133664 title "Enhanced GLT-1 mediated glutamate uptake and migration of primary astrocytes directed by fibronectin-coated electrospun poly-l-lactic acid fibers" @default.
• W2079133664 cites W1966183236 @default.
• W2079133664 cites W1966610248 @default.
• W2079133664 cites W1969212735 @default.
• W2079133664 cites W1970906375 @default.
• W2079133664 cites W1975719696 @default.
• W2079133664 cites W1978892749 @default.
• W2079133664 cites W1979812031 @default.
• W2079133664 cites W1987080089 @default.
• W2079133664 cites W1990078383 @default.
• W2079133664 cites W1996422388 @default.
• W2079133664 cites W2005327608 @default.
• W2079133664 cites W2008720843 @default.
• W2079133664 cites W2010891842 @default.
• W2079133664 cites W2015541518 @default.
• W2079133664 cites W2016616421 @default.
• W2079133664 cites W2022345246 @default.
• W2079133664 cites W2024405375 @default.
• W2079133664 cites W2027393834 @default.
• W2079133664 cites W2028564194 @default.
• W2079133664 cites W2031023695 @default.
• W2079133664 cites W2040840739 @default.
• W2079133664 cites W2050470032 @default.
• W2079133664 cites W2057358727 @default.
• W2079133664 cites W2063602901 @default.
• W2079133664 cites W2065663423 @default.
• W2079133664 cites W2070268956 @default.
• W2079133664 cites W2074571304 @default.
• W2079133664 cites W2074661552 @default.
• W2079133664 cites W2076577863 @default.
• W2079133664 cites W2078456821 @default.
• W2079133664 cites W2078694016 @default.
• W2079133664 cites W2080203024 @default.
• W2079133664 cites W2081225544 @default.
• W2079133664 cites W2087131768 @default.
• W2079133664 cites W2087609924 @default.
• W2079133664 cites W2095931805 @default.
• W2079133664 cites W2100169867 @default.
• W2079133664 cites W2103441049 @default.
• W2079133664 cites W2104968232 @default.
• W2079133664 cites W2127722806 @default.
• W2079133664 cites W2128744963 @default.
• W2079133664 cites W2138240749 @default.
• W2079133664 cites W2139748951 @default.
• W2079133664 cites W2157853053 @default.
• W2079133664 cites W2160935383 @default.
• W2079133664 cites W2163234334 @default.
• W2079133664 cites W2165287397 @default.
• W2079133664 cites W2169384696 @default.
• W2079133664 cites W4211037293 @default.
• W2079133664 doi "https://doi.org/10.1016/j.biomaterials.2013.10.079" @default.
• W2079133664 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4183153" @default.
• W2079133664 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24246642" @default.
• W2079133664 hasPublicationYear "2014" @default.
• W2079133664 type Work @default.
• W2079133664 sameAs 2079133664 @default.
• W2079133664 citedByCount "83" @default.
• W2079133664 countsByYear W20791336642014 @default.
• W2079133664 countsByYear W20791336642015 @default.
• W2079133664 countsByYear W20791336642016 @default.
• W2079133664 countsByYear W20791336642017 @default.
• W2079133664 countsByYear W20791336642018 @default.
• W2079133664 countsByYear W20791336642019 @default.
• W2079133664 countsByYear W20791336642020 @default.
• W2079133664 countsByYear W20791336642021 @default.
• W2079133664 countsByYear W20791336642022 @default.
• W2079133664 countsByYear W20791336642023 @default.
• W2079133664 crossrefType "journal-article" @default.
• W2079133664 hasAuthorship W2079133664A5015163321 @default.
• W2079133664 hasAuthorship W2079133664A5024938105 @default.
• W2079133664 hasAuthorship W2079133664A5046498224 @default.
• W2079133664 hasAuthorship W2079133664A5077395570 @default.
• W2079133664 hasAuthorship W2079133664A5081624863 @default.
• W2079133664 hasAuthorship W2079133664A5085880004 @default.
• W2079133664 hasAuthorship W2079133664A5090293126 @default.
• W2079133664 hasBestOaLocation W20791336642 @default.
• W2079133664 hasConcept C12554922 @default.
• W2079133664 hasConcept C142669718 @default.
• W2079133664 hasConcept C144796933 @default.
• W2079133664 hasConcept C1491633281 @default.
• W2079133664 hasConcept C159985019 @default.
• W2079133664 hasConcept C169760540 @default.
• W2079133664 hasConcept C170493617 @default.
• W2079133664 hasConcept C189040839 @default.
• W2079133664 hasConcept C189165786 @default.
• W2079133664 hasConcept C192562407 @default.
• W2079133664 hasConcept C2777542381 @default.

• next