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- W2079157435 abstract "Purpose To investigate the expression and distribution of Wnt pathway antagonists, secreted frizzled-related proteins (SFRPs), in tears and corneas from control and keratoconus (KC) patients. Methods Immunofluorescence and confocal microscopy were used to examine the expression patterns of SFRP1, 2, 3, and 5 in paraffin sections of KC (n=11) and control (n=7) corneas. A custom SFRP1 ELISA was developed and used to quantify SFRP1 concentration in basal tear samples collected from KC (n=10) and control patients (n=10). The percentage SFRP1 of total tear protein concentration (%SFRP1) was calculated and compared between KC and control groups. Results Immunofluorescence showed heterogeneous epithelial SFRP1 expression in all KC corneas compared to low expression in controls. SFRP3 and SFRP5 showed unique patterns of immunolabelling. SFRP3 was localised to epithelial cell membranes and limbal vessels in control corneas; cell membrane and cytoplasmic immunolabelling, and increased expression centrally, was seen in KC. SFRP5 was expressed strongly in the corneal stroma (KC and control). SFRP2 immunoreactivity not obvious in either control or KC corneas. ELISA results showed that tear SFRP1 levels were lower in KC compared to control tears. Conclusion The pathogenesis of KC remains poorly understood. Our current study shows that SFRP1, 2, 3 and 5 are differentially expressed in control and KC corneas, consistent with previous work, suggesting Wnt signalling involvement in KC. The decreased levels of KC tear SFRP1 contrasts with increased expression in KC corneal epithelium, and may suggest KC-associated differences in tear secretion or tear protein breakdown. Funded in part by Sydney Eye Hospital Foundation" @default.
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- W2079157435 date "2012-08-06" @default.
- W2079157435 modified "2023-09-27" @default.
- W2079157435 title "Secreted frizzled proteins in control and keratoconus (KC) tears and corneas" @default.
- W2079157435 doi "https://doi.org/10.1111/j.1755-3768.2012.4447.x" @default.
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