Matches in SemOpenAlex for { <https://semopenalex.org/work/W2079164590> ?p ?o ?g. }
Showing items 1 to 85 of
85
with 100 items per page.
- W2079164590 endingPage "1491" @default.
- W2079164590 startingPage "1485" @default.
- W2079164590 abstract "Alpha-galactosidase was purified from a fresh fruiting body of Ganoderma lucidum by precipitation with ammonium sulfate and column chromatographies with DEAE-Sephadex and Con A-Sepharose. The purified enzyme was homogeneous on polyacrylamide gel electrophoresis. Its N-terminal amino acid sequence was similar to that of Mortierella vinacea alpha-galactosidase. The molecular mass of the enzyme was about 56 kDa by SDS-polyacrylamide gel electrophoresis, and about 249 kDa by gel filtration column chromatography. The optimum pH and temperature were 6.0 and 70 degrees C, respectively. The enzyme was fully stable to heating at 70 degrees C for 30 min. It hydrolyzed p-nitrophenyl-alpha-D-galactopyranoside (Km=0.4 mM) but hydrolyzed little o-nitrophenyl-alpha-D-galactopyranoside. It also hydrolyzed melibiose, raffinose, and stachyose. The enzyme catalyzed the transgalactosylation reaction which synthesized melibiose. The product was confirmed by various analyses." @default.
- W2079164590 created "2016-06-24" @default.
- W2079164590 creator A5042691162 @default.
- W2079164590 creator A5043688508 @default.
- W2079164590 creator A5044393217 @default.
- W2079164590 creator A5068552779 @default.
- W2079164590 creator A5080727519 @default.
- W2079164590 date "2003-01-01" @default.
- W2079164590 modified "2023-09-26" @default.
- W2079164590 title "Purification and Characterization of Thermostable α-Galactosidase from<i>Ganoderma lucidum</i>" @default.
- W2079164590 cites W1531902268 @default.
- W2079164590 doi "https://doi.org/10.1271/bbb.67.1485" @default.
- W2079164590 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12913291" @default.
- W2079164590 hasPublicationYear "2003" @default.
- W2079164590 type Work @default.
- W2079164590 sameAs 2079164590 @default.
- W2079164590 citedByCount "40" @default.
- W2079164590 countsByYear W20791645902012 @default.
- W2079164590 countsByYear W20791645902013 @default.
- W2079164590 countsByYear W20791645902014 @default.
- W2079164590 countsByYear W20791645902015 @default.
- W2079164590 countsByYear W20791645902016 @default.
- W2079164590 countsByYear W20791645902017 @default.
- W2079164590 countsByYear W20791645902018 @default.
- W2079164590 countsByYear W20791645902019 @default.
- W2079164590 countsByYear W20791645902020 @default.
- W2079164590 countsByYear W20791645902022 @default.
- W2079164590 crossrefType "journal-article" @default.
- W2079164590 hasAuthorship W2079164590A5042691162 @default.
- W2079164590 hasAuthorship W2079164590A5043688508 @default.
- W2079164590 hasAuthorship W2079164590A5044393217 @default.
- W2079164590 hasAuthorship W2079164590A5068552779 @default.
- W2079164590 hasAuthorship W2079164590A5080727519 @default.
- W2079164590 hasConcept C101660505 @default.
- W2079164590 hasConcept C116084860 @default.
- W2079164590 hasConcept C163994747 @default.
- W2079164590 hasConcept C181199279 @default.
- W2079164590 hasConcept C185592680 @default.
- W2079164590 hasConcept C193363816 @default.
- W2079164590 hasConcept C2776428949 @default.
- W2079164590 hasConcept C2778139943 @default.
- W2079164590 hasConcept C2778518025 @default.
- W2079164590 hasConcept C2778597767 @default.
- W2079164590 hasConcept C2779998883 @default.
- W2079164590 hasConcept C43617362 @default.
- W2079164590 hasConcept C55493867 @default.
- W2079164590 hasConcept C58121356 @default.
- W2079164590 hasConcept C94412978 @default.
- W2079164590 hasConceptScore W2079164590C101660505 @default.
- W2079164590 hasConceptScore W2079164590C116084860 @default.
- W2079164590 hasConceptScore W2079164590C163994747 @default.
- W2079164590 hasConceptScore W2079164590C181199279 @default.
- W2079164590 hasConceptScore W2079164590C185592680 @default.
- W2079164590 hasConceptScore W2079164590C193363816 @default.
- W2079164590 hasConceptScore W2079164590C2776428949 @default.
- W2079164590 hasConceptScore W2079164590C2778139943 @default.
- W2079164590 hasConceptScore W2079164590C2778518025 @default.
- W2079164590 hasConceptScore W2079164590C2778597767 @default.
- W2079164590 hasConceptScore W2079164590C2779998883 @default.
- W2079164590 hasConceptScore W2079164590C43617362 @default.
- W2079164590 hasConceptScore W2079164590C55493867 @default.
- W2079164590 hasConceptScore W2079164590C58121356 @default.
- W2079164590 hasConceptScore W2079164590C94412978 @default.
- W2079164590 hasIssue "7" @default.
- W2079164590 hasLocation W20791645901 @default.
- W2079164590 hasLocation W20791645902 @default.
- W2079164590 hasOpenAccess W2079164590 @default.
- W2079164590 hasPrimaryLocation W20791645901 @default.
- W2079164590 hasRelatedWork W1560742689 @default.
- W2079164590 hasRelatedWork W2001321907 @default.
- W2079164590 hasRelatedWork W2041969841 @default.
- W2079164590 hasRelatedWork W2062594954 @default.
- W2079164590 hasRelatedWork W2079164590 @default.
- W2079164590 hasRelatedWork W2144832700 @default.
- W2079164590 hasRelatedWork W2189485424 @default.
- W2079164590 hasRelatedWork W2382010943 @default.
- W2079164590 hasRelatedWork W2515447035 @default.
- W2079164590 hasRelatedWork W4244968863 @default.
- W2079164590 hasVolume "67" @default.
- W2079164590 isParatext "false" @default.
- W2079164590 isRetracted "false" @default.
- W2079164590 magId "2079164590" @default.
- W2079164590 workType "article" @default.