FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2079169610> ?p ?o ?g. }

• W2079169610 endingPage "811" @default.
• W2079169610 startingPage "806" @default.
• W2079169610 abstract "Abstract Background Use of retinoic acid (RA) has become the standard of care in the treatment of high risk neuroblastoma (NB). In vitro, RA induces growth arrest and differentiation, an effect that likely underlies its activity in the clinical setting. An important event in differentiation is the transcriptional downregulation of the MYCN oncogene, which is frequently activated in aggressive tumors. While it is known that Sp1/Sp3 and E2F are necessary to drive basal MYCN expression, the mechanism for its downregulation by RA remains enigmatic. Changes in E2F binding have been reported, however these occurred after the actual transcriptional response. Here, post‐translational modifications of Sp proteins were examined as an alternate mechanism of RA‐mediated promoter regulation. Procedure Western blot was used to evaluate steady state levels of nuclear/cytoplasmic Sp1/Sp3. Promoter binding and DNA conformation were determined by gel shift, circular permutation, and chromatin immunoprecipitation assays. Immunoprecipitation/western and 32 P‐phosphoamino analyses were used to detect glycosylation, acetylation, sumoylation, and phosphorylation. Results RA did not affect the cellular level of Sp1/Sp3 proteins, their nuclear/cytoplasmic distribution, ability to bind the MYCN promoter, degree of Sp‐induced DNA bending, or post‐translational modifications. Conclusions MYCN RA response is not mediated solely though the region controlling basal activity. RA may be exerting its effects via multiple non‐adjacent regulatory regions, potentially including basal motifs, either within the MYCN promoter or distally, on the same or even different chromosomes. Such cooperative trans‐type DNA–protein interactions could explain the inaccessibility of this mechanism to the locus‐specific approaches employed up to this point. Pediatr Blood Cancer 2008;50:806–811. © 2007 Wiley‐Liss, Inc." @default.
• W2079169610 created "2016-06-24" @default.
• W2079169610 creator A5005620104 @default.
• W2079169610 creator A5021012824 @default.
• W2079169610 creator A5024539701 @default.
• W2079169610 creator A5067946696 @default.
• W2079169610 creator A5071755934 @default.
• W2079169610 date "2007-06-06" @default.
• W2079169610 modified "2023-10-18" @default.
• W2079169610 title "Retinoic acid induced downregulation of MYCN is not mediated through changes in Sp1/Sp3" @default.
• W2079169610 cites W1808154214 @default.
• W2079169610 cites W18221114 @default.
• W2079169610 cites W1857381743 @default.
• W2079169610 cites W1969415535 @default.
• W2079169610 cites W1993243592 @default.
• W2079169610 cites W1999424147 @default.
• W2079169610 cites W2005999235 @default.
• W2079169610 cites W2010425964 @default.
• W2079169610 cites W2011467252 @default.
• W2079169610 cites W2024412859 @default.
• W2079169610 cites W2025577759 @default.
• W2079169610 cites W2039536163 @default.
• W2079169610 cites W2047749014 @default.
• W2079169610 cites W2051663421 @default.
• W2079169610 cites W2057364464 @default.
• W2079169610 cites W2062175667 @default.
• W2079169610 cites W2064428081 @default.
• W2079169610 cites W2067606868 @default.
• W2079169610 cites W2074356752 @default.
• W2079169610 cites W2074456375 @default.
• W2079169610 cites W2074469807 @default.
• W2079169610 cites W2078213867 @default.
• W2079169610 cites W2078331020 @default.
• W2079169610 cites W2086438431 @default.
• W2079169610 cites W2087296700 @default.
• W2079169610 cites W2094105923 @default.
• W2079169610 cites W2099449144 @default.
• W2079169610 cites W2103184621 @default.
• W2079169610 cites W2114964602 @default.
• W2079169610 cites W2117909268 @default.
• W2079169610 cites W2121091839 @default.
• W2079169610 cites W2132855064 @default.
• W2079169610 cites W2160494443 @default.
• W2079169610 cites W2168380993 @default.
• W2079169610 cites W2326525648 @default.
• W2079169610 cites W2327522594 @default.
• W2079169610 doi "https://doi.org/10.1002/pbc.21273" @default.
• W2079169610 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17554788" @default.
• W2079169610 hasPublicationYear "2007" @default.
• W2079169610 type Work @default.
• W2079169610 sameAs 2079169610 @default.
• W2079169610 citedByCount "7" @default.
• W2079169610 countsByYear W20791696102012 @default.
• W2079169610 countsByYear W20791696102014 @default.
• W2079169610 countsByYear W20791696102021 @default.
• W2079169610 crossrefType "journal-article" @default.
• W2079169610 hasAuthorship W2079169610A5005620104 @default.
• W2079169610 hasAuthorship W2079169610A5021012824 @default.
• W2079169610 hasAuthorship W2079169610A5024539701 @default.
• W2079169610 hasAuthorship W2079169610A5067946696 @default.
• W2079169610 hasAuthorship W2079169610A5071755934 @default.
• W2079169610 hasConcept C101762097 @default.
• W2079169610 hasConcept C104317684 @default.
• W2079169610 hasConcept C119157956 @default.
• W2079169610 hasConcept C127561419 @default.
• W2079169610 hasConcept C134320426 @default.
• W2079169610 hasConcept C150194340 @default.
• W2079169610 hasConcept C153911025 @default.
• W2079169610 hasConcept C27153228 @default.
• W2079169610 hasConcept C2779634854 @default.
• W2079169610 hasConcept C2781121885 @default.
• W2079169610 hasConcept C502942594 @default.
• W2079169610 hasConcept C54355233 @default.
• W2079169610 hasConcept C71829478 @default.
• W2079169610 hasConcept C71924100 @default.
• W2079169610 hasConcept C74401373 @default.
• W2079169610 hasConcept C83258922 @default.
• W2079169610 hasConcept C86339819 @default.
• W2079169610 hasConcept C86803240 @default.
• W2079169610 hasConcept C95444343 @default.
• W2079169610 hasConceptScore W2079169610C101762097 @default.
• W2079169610 hasConceptScore W2079169610C104317684 @default.
• W2079169610 hasConceptScore W2079169610C119157956 @default.
• W2079169610 hasConceptScore W2079169610C127561419 @default.
• W2079169610 hasConceptScore W2079169610C134320426 @default.
• W2079169610 hasConceptScore W2079169610C150194340 @default.
• W2079169610 hasConceptScore W2079169610C153911025 @default.
• W2079169610 hasConceptScore W2079169610C27153228 @default.
• W2079169610 hasConceptScore W2079169610C2779634854 @default.
• W2079169610 hasConceptScore W2079169610C2781121885 @default.
• W2079169610 hasConceptScore W2079169610C502942594 @default.
• W2079169610 hasConceptScore W2079169610C54355233 @default.
• W2079169610 hasConceptScore W2079169610C71829478 @default.
• W2079169610 hasConceptScore W2079169610C71924100 @default.
• W2079169610 hasConceptScore W2079169610C74401373 @default.
• W2079169610 hasConceptScore W2079169610C83258922 @default.
• W2079169610 hasConceptScore W2079169610C86339819 @default.
• W2079169610 hasConceptScore W2079169610C86803240 @default.

• next