Matches in SemOpenAlex for { <https://semopenalex.org/work/W2079170463> ?p ?o ?g. }
- W2079170463 endingPage "598" @default.
- W2079170463 startingPage "583" @default.
- W2079170463 abstract "We have recently shown that actin can be modified by the Michael addition of 4-hydroxynonenal to Cys374. Here, we have exposed purified actin at increasing acrolein concentrations and have identified the sites of acrolein addition using LC-ESI-MS/MS. Acrolein reacted with Cys374, His87, His173, and, minimally, His40. Cys374 adduction by both 4-hydroxynonenal and acrolein negligibly affected the polymerization of aldehyde-modified (carbonylated) actin, as shown by fluorescence measurements. Differently, acrolein binding at histidine residues, when Cys374 was completely saturated, inhibited polymerization in a dose-dependent manner. Molecular modeling analyses indicated that structural distortions of the ATP-binding site, induced by four acrolein-Michael adducts, could explain the changes in the polymerization process. Aldehyde binding to Cys374 does not alter significantly actin polymerization because this residue is located in a very flexible region, whose covalent modifications do not alter the protein folding. These data demonstrate that Cys374 represents the primary target site of α,β-unsaturated aldehyde addition to actin in vitro. As Cys374 is a preferential target for various oxidative/nitrosative modifications, and actin is one of the main carbonylated proteins in vivo, these findings also suggest that the highly reactive Cys374 could serve as a carbonyl scavenger of reactive α,β-unsaturated aldehydes and other electrophilic lipids." @default.
- W2079170463 created "2016-06-24" @default.
- W2079170463 creator A5001799610 @default.
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- W2079170463 creator A5021943550 @default.
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- W2079170463 creator A5069021426 @default.
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- W2079170463 creator A5085900234 @default.
- W2079170463 date "2007-03-01" @default.
- W2079170463 modified "2023-10-18" @default.
- W2079170463 title "Actin Cys374 as a nucleophilic target of α,β-unsaturated aldehydes" @default.
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