Matches in SemOpenAlex for { <https://semopenalex.org/work/W2079181298> ?p ?o ?g. }
Showing items 1 to 75 of
75
with 100 items per page.
- W2079181298 endingPage "319" @default.
- W2079181298 startingPage "314" @default.
- W2079181298 abstract "Abstract Intradermal skin testing may often give inaccurate results because of poor stability of allergens and loss of protein by adsorption to the walls of containers and syringes during the process of making the extreme dilutions required with potent extracts. To test the ability of stabilizers to prevent such losses of allergenic activity, three diluents for allergens were compared: standard phosphate-buffered saline (PBS), pH 7.4, containing 0.4% phenol and the same buffer containing either 0.03% human serum albumin (HSA) or 0.005% Tween 80. Tenfold dilution series of ragweed, grass, Altemaria , and dust allergens were tested by the intradermal threshold dilution technique in the same group of patients five times over six months, comparing stored dilutions with dilutions freshly made from the same batches of lyophilized extracts. Results with Tween 80 and HSA buffers were identical and highly reproducible; however, each new set of dilutions in standard buffers frequently showed within 24 to 48 hr after preparation a lower skin test potency which varied unpredictably between ten and one thousandfold. Furthermore, upon prolonged storage at 4 °C, dilutions in standard buffer lost further activity. Storage of radiolabeled antigen E (AgE) in ordinary glass tubes for 24 hr showed adsorption of about 5% of the labeled protein to glass in the absence of stabilizers but only 0.5% in the presence of stabilizers. We conclude that stabilizing agents should be added to diluting fluids in preparing allergens for skin testing or immunotherapy." @default.
- W2079181298 created "2016-06-24" @default.
- W2079181298 creator A5049027996 @default.
- W2079181298 creator A5054711386 @default.
- W2079181298 date "1978-11-01" @default.
- W2079181298 modified "2023-09-25" @default.
- W2079181298 title "Human serum albumin and Tween 80 as stabilizers of allergen solutions" @default.
- W2079181298 cites W1561166329 @default.
- W2079181298 cites W1974766261 @default.
- W2079181298 cites W2020545328 @default.
- W2079181298 cites W2055112026 @default.
- W2079181298 cites W2073083356 @default.
- W2079181298 cites W2088517413 @default.
- W2079181298 cites W2092382573 @default.
- W2079181298 cites W2111937371 @default.
- W2079181298 cites W4247850342 @default.
- W2079181298 doi "https://doi.org/10.1016/0091-6749(78)90164-1" @default.
- W2079181298 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/701667" @default.
- W2079181298 hasPublicationYear "1978" @default.
- W2079181298 type Work @default.
- W2079181298 sameAs 2079181298 @default.
- W2079181298 citedByCount "41" @default.
- W2079181298 countsByYear W20791812982014 @default.
- W2079181298 countsByYear W20791812982016 @default.
- W2079181298 countsByYear W20791812982019 @default.
- W2079181298 countsByYear W20791812982020 @default.
- W2079181298 countsByYear W20791812982021 @default.
- W2079181298 crossrefType "journal-article" @default.
- W2079181298 hasAuthorship W2079181298A5049027996 @default.
- W2079181298 hasAuthorship W2079181298A5054711386 @default.
- W2079181298 hasBestOaLocation W20791812981 @default.
- W2079181298 hasConcept C165616093 @default.
- W2079181298 hasConcept C185592680 @default.
- W2079181298 hasConcept C203014093 @default.
- W2079181298 hasConcept C207480886 @default.
- W2079181298 hasConcept C2776125364 @default.
- W2079181298 hasConcept C2778597219 @default.
- W2079181298 hasConcept C2780510475 @default.
- W2079181298 hasConcept C2991920594 @default.
- W2079181298 hasConcept C43617362 @default.
- W2079181298 hasConcept C55493867 @default.
- W2079181298 hasConcept C71924100 @default.
- W2079181298 hasConceptScore W2079181298C165616093 @default.
- W2079181298 hasConceptScore W2079181298C185592680 @default.
- W2079181298 hasConceptScore W2079181298C203014093 @default.
- W2079181298 hasConceptScore W2079181298C207480886 @default.
- W2079181298 hasConceptScore W2079181298C2776125364 @default.
- W2079181298 hasConceptScore W2079181298C2778597219 @default.
- W2079181298 hasConceptScore W2079181298C2780510475 @default.
- W2079181298 hasConceptScore W2079181298C2991920594 @default.
- W2079181298 hasConceptScore W2079181298C43617362 @default.
- W2079181298 hasConceptScore W2079181298C55493867 @default.
- W2079181298 hasConceptScore W2079181298C71924100 @default.
- W2079181298 hasIssue "5" @default.
- W2079181298 hasLocation W20791812981 @default.
- W2079181298 hasLocation W20791812982 @default.
- W2079181298 hasOpenAccess W2079181298 @default.
- W2079181298 hasPrimaryLocation W20791812981 @default.
- W2079181298 hasRelatedWork W1988290961 @default.
- W2079181298 hasRelatedWork W2050537417 @default.
- W2079181298 hasRelatedWork W2052974877 @default.
- W2079181298 hasRelatedWork W2144443144 @default.
- W2079181298 hasRelatedWork W2155569634 @default.
- W2079181298 hasRelatedWork W2354779584 @default.
- W2079181298 hasRelatedWork W2355403441 @default.
- W2079181298 hasRelatedWork W2399993072 @default.
- W2079181298 hasRelatedWork W2408134454 @default.
- W2079181298 hasRelatedWork W4313372676 @default.
- W2079181298 hasVolume "62" @default.
- W2079181298 isParatext "false" @default.
- W2079181298 isRetracted "false" @default.
- W2079181298 magId "2079181298" @default.
- W2079181298 workType "article" @default.