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• W2079182964 abstract "Cryopreservation plays a key role in the long-term storage of native and engineered cells and tissues for research and clinical applications. The survival of cells and tissues after freezing and thawing depends on the ability of the cells to withstand a variety of stresses imposed by the cryopreservation protocol. A better understanding of the nature and kinetics of cellular responses to temperature-induced conditions is required to minimize cryoinjury. An interrupted freezing procedure that allows dissection of cryoinjury was used to investigate the progressive damage that occurs to cells during cryopreservation using slow cooling. Simulations of cellular osmotic responses were used to provide interpretation linking states of the cell with events during the freezing procedure. Simulations of graded freezing (interrupted slow cooling without hold time) were correlated with cell recovery results of TF-1 cells. Calculated intracellular supercooling and osmolality, were used as indicators of the probability of cryoinjury due to intracellular ice formation and solution effects, providing direct links of cellular conditions to events in the freezing process. Using simulations, this study demonstrated that both intracellular supercooling and osmolality are necessary to explain graded freezing results." @default.
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• W2079182964 date "2010-08-01" @default.
• W2079182964 modified "2023-09-24" @default.
• W2079182964 title "Investigating cryoinjury using simulations and experiments: 2. TF-1 cells during graded freezing (interrupted slow cooling without hold time)" @default.
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• W2079182964 doi "https://doi.org/10.1016/j.cryobiol.2010.04.005" @default.
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