Matches in SemOpenAlex for { <https://semopenalex.org/work/W2079183268> ?p ?o ?g. }
- W2079183268 endingPage "651" @default.
- W2079183268 startingPage "644" @default.
- W2079183268 abstract "Stem cells have received a great deal of attention for their clinical and therapeutic potential for treating human diseases and disorders. Recent studies have shown that it is possible to genetically engineered stem cells (GESTECs) to produce suicide enzymes that convert non-toxic prodrugs to toxic metabolites, selectively migrate toward tumor sites and reduce tumor growth. In this study, we evaluated whether these GESTECs are capable of migrating to hepatocarcinoma cells and examined the potential therapeutic efficacy of gene-directed enzyme prodrug therapy against liver cancer cells in cellular and animal models. A modified transwell migration assay was performed to determine the migratory capacity of GESTECs to Hep3B hepatocarcinoma cells. GESTECs, that is, HB1.F3.CD or HB1.F3.CD.interferon-β (IFN-β) cells, engineered to express a suicide gene, cytosine deaminase (CD), selectively migrated toward liver cancer cells. Treatment of Hep3B, human liver cancer cells, with the prodrug 5-fluorocytosine (5-FC) in the presence of HB1.F3.CD or HB1.F3.CD.IFN-β cells resulted in the inhibition of Hep3B cell growth. In a xenografted mouse model injected with hepatocarcinoma, we investigated the therapeutic effect of these stem cells. For 9 weeks, the xenografted mice were treated with HB1.F3.CD or HB1.F3.CD.IFN-β in the presence of 5-FC. A growth of tumor mass was inhibited about 40–50% in the mice treated with GESTECs and a prodrug. In addition, we further confirmed the cytotoxic effect on tumor cells by histological analysis and migratory effect of therapeutic stem cells. Taken together, GESTECs expressing a fusion gene encoding CD and IFN-β may exert a synergistic antitumor effect on this type of tumor." @default.
- W2079183268 created "2016-06-24" @default.
- W2079183268 creator A5024635161 @default.
- W2079183268 creator A5031112476 @default.
- W2079183268 creator A5037196403 @default.
- W2079183268 creator A5038864663 @default.
- W2079183268 creator A5067427651 @default.
- W2079183268 creator A5070989428 @default.
- W2079183268 creator A5073092107 @default.
- W2079183268 date "2012-07-13" @default.
- W2079183268 modified "2023-10-18" @default.
- W2079183268 title "Synergistic effects of genetically engineered stem cells expressing cytosine deaminase and interferon-β via their tumor tropism to selectively target human hepatocarcinoma cells" @default.
- W2079183268 cites W1828890888 @default.
- W2079183268 cites W1975503701 @default.
- W2079183268 cites W1976657370 @default.
- W2079183268 cites W1980441719 @default.
- W2079183268 cites W1993252085 @default.
- W2079183268 cites W1995730494 @default.
- W2079183268 cites W2002868143 @default.
- W2079183268 cites W2004356764 @default.
- W2079183268 cites W2009705590 @default.
- W2079183268 cites W2017593380 @default.
- W2079183268 cites W2020830322 @default.
- W2079183268 cites W2024490785 @default.
- W2079183268 cites W2041745928 @default.
- W2079183268 cites W2043951860 @default.
- W2079183268 cites W2049532316 @default.
- W2079183268 cites W2056056608 @default.
- W2079183268 cites W2061904165 @default.
- W2079183268 cites W2062128964 @default.
- W2079183268 cites W2063892111 @default.
- W2079183268 cites W2069539177 @default.
- W2079183268 cites W2070823657 @default.
- W2079183268 cites W2072246632 @default.
- W2079183268 cites W2097942616 @default.
- W2079183268 cites W2099886521 @default.
- W2079183268 cites W2103283961 @default.
- W2079183268 cites W2103637700 @default.
- W2079183268 cites W2121590586 @default.
- W2079183268 cites W2127771136 @default.
- W2079183268 cites W2127823872 @default.
- W2079183268 cites W2133867101 @default.
- W2079183268 cites W2135212691 @default.
- W2079183268 cites W2137846955 @default.
- W2079183268 cites W2141652044 @default.
- W2079183268 cites W2145630676 @default.
- W2079183268 cites W2367941480 @default.
- W2079183268 cites W3189093243 @default.
- W2079183268 cites W4233423789 @default.
- W2079183268 doi "https://doi.org/10.1038/cgt.2012.45" @default.
- W2079183268 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22790964" @default.
- W2079183268 hasPublicationYear "2012" @default.
- W2079183268 type Work @default.
- W2079183268 sameAs 2079183268 @default.
- W2079183268 citedByCount "19" @default.
- W2079183268 countsByYear W20791832682013 @default.
- W2079183268 countsByYear W20791832682014 @default.
- W2079183268 countsByYear W20791832682015 @default.
- W2079183268 countsByYear W20791832682016 @default.
- W2079183268 countsByYear W20791832682017 @default.
- W2079183268 countsByYear W20791832682018 @default.
- W2079183268 countsByYear W20791832682019 @default.
- W2079183268 countsByYear W20791832682023 @default.
- W2079183268 crossrefType "journal-article" @default.
- W2079183268 hasAuthorship W2079183268A5024635161 @default.
- W2079183268 hasAuthorship W2079183268A5031112476 @default.
- W2079183268 hasAuthorship W2079183268A5037196403 @default.
- W2079183268 hasAuthorship W2079183268A5038864663 @default.
- W2079183268 hasAuthorship W2079183268A5067427651 @default.
- W2079183268 hasAuthorship W2079183268A5070989428 @default.
- W2079183268 hasAuthorship W2079183268A5073092107 @default.
- W2079183268 hasBestOaLocation W20791832681 @default.
- W2079183268 hasConcept C104317684 @default.
- W2079183268 hasConcept C108215921 @default.
- W2079183268 hasConcept C111599444 @default.
- W2079183268 hasConcept C121608353 @default.
- W2079183268 hasConcept C153911025 @default.
- W2079183268 hasConcept C154317977 @default.
- W2079183268 hasConcept C185592680 @default.
- W2079183268 hasConcept C202751555 @default.
- W2079183268 hasConcept C203014093 @default.
- W2079183268 hasConcept C2776178377 @default.
- W2079183268 hasConcept C2777748644 @default.
- W2079183268 hasConcept C28328180 @default.
- W2079183268 hasConcept C34981463 @default.
- W2079183268 hasConcept C502942594 @default.
- W2079183268 hasConcept C54355233 @default.
- W2079183268 hasConcept C55493867 @default.
- W2079183268 hasConcept C86803240 @default.
- W2079183268 hasConcept C95444343 @default.
- W2079183268 hasConcept C96232424 @default.
- W2079183268 hasConcept C98274493 @default.
- W2079183268 hasConceptScore W2079183268C104317684 @default.
- W2079183268 hasConceptScore W2079183268C108215921 @default.
- W2079183268 hasConceptScore W2079183268C111599444 @default.
- W2079183268 hasConceptScore W2079183268C121608353 @default.
- W2079183268 hasConceptScore W2079183268C153911025 @default.
- W2079183268 hasConceptScore W2079183268C154317977 @default.