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- W2079183751 endingPage "2726" @default.
- W2079183751 startingPage "2712" @default.
- W2079183751 abstract "Nuclear precursor mRNA splicing requires the stepwise assembly of a large complex, the spliceosome. Recent large-scale analyses, including purification of splicing complexes, high-throughput genetic screens and interactomic studies, have linked numerous factors to this dynamic process, including a well-defined core conserved from yeast to human. Intriguingly, despite extensive studies, no splicing defects were reported for some of the corresponding yeast mutants. To resolve this paradox, we screened a collection of viable yeast strains carrying mutations in splicing-related factors with a set of reporters including artificial constructs carrying competing splice sites. Previous analyses have indeed demonstrated that this strategy identifies yeast factors able to regulate alternative splicing and whose properties are conserved in human cells. The method, sensitive to subtle defects, revealed new splicing phenotypes for most analyzed factors such as the Urn1 protein. Interestingly, a mutant of PRP8 specifically lacking an N-terminal proline-rich region stimulated the splicing of a reporter containing competing branchpoint/3′ splice site regions. Thus, using appropriate reporters, yeast can be used to quickly delineate the effect of various factors on splicing and identify those with the propensity to regulate alternative splicing events. Structured digital abstract Lsm8 physically interacts with Prp4, Prp24, Prp3, Prp6, Snu114, Brr2, Lsm4 and Prp8 by tandem affinity purification (View interaction)" @default.
- W2079183751 created "2016-06-24" @default.
- W2079183751 creator A5023117880 @default.
- W2079183751 creator A5079371539 @default.
- W2079183751 date "2013-05-09" @default.
- W2079183751 modified "2023-10-13" @default.
- W2079183751 title "Rapid screening of yeast mutants with reporters identifies new splicing phenotypes" @default.
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- W2079183751 doi "https://doi.org/10.1111/febs.12277" @default.
- W2079183751 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23560879" @default.