Matches in SemOpenAlex for { <https://semopenalex.org/work/W2079185366> ?p ?o ?g. }
- W2079185366 endingPage "e64644" @default.
- W2079185366 startingPage "e64644" @default.
- W2079185366 abstract "Clostridium perfringens β-toxin (CPB) is a β-barrel pore-forming toxin and an essential virulence factor of C. perfringens type C strains, which cause fatal hemorrhagic enteritis in animals and humans. We have previously shown that CPB is bound to endothelial cells within the intestine of affected pigs and humans, and that CPB is highly toxic to primary porcine endothelial cells (pEC) in vitro. The objective of the present study was to investigate the type of cell death induced by CPB in these cells, and to study potential host cell mechanisms involved in this process. CPB rapidly induced lactate dehydrogenase (LDH) release, propidium iodide uptake, ATP depletion, potassium efflux, a marked rise in intracellular calcium [Ca2+]i, release of high-mobility group protein B1 (HMGB1), and caused ultrastructural changes characteristic of necrotic cell death. Despite a certain level of caspase-3 activation, no appreciable DNA fragmentation was detected. CPB-induced LDH release and propidium iodide uptake were inhibited by necrostatin-1 and the two dissimilar calpain inhibitors PD150606 and calpeptin. Likewise, inhibition of potassium efflux, chelation of intracellular calcium and treatment of pEC with cyclosporin A also significantly inhibited CPB-induced LDH release. Our results demonstrate that rCPB primarily induces necrotic cell death in pEC, and that necrotic cell death is not merely a passive event caused by toxin-induced membrane disruption, but is propagated by host cell-dependent biochemical pathways activated by the rise in intracellular calcium and inhibitable by necrostatin-1, consistent with the emerging concept of programmed necrosis (“necroptosis”)." @default.
- W2079185366 created "2016-06-24" @default.
- W2079185366 creator A5007069708 @default.
- W2079185366 creator A5019497452 @default.
- W2079185366 creator A5060358338 @default.
- W2079185366 creator A5060420474 @default.
- W2079185366 creator A5082178462 @default.
- W2079185366 creator A5091893555 @default.
- W2079185366 date "2013-05-29" @default.
- W2079185366 modified "2023-09-27" @default.
- W2079185366 title "Clostridium perfringens Beta-Toxin Induces Necrostatin-Inhibitable, Calpain-Dependent Necrosis in Primary Porcine Endothelial Cells" @default.
- W2079185366 cites W1498214333 @default.
- W2079185366 cites W1829313968 @default.
- W2079185366 cites W1933260492 @default.
- W2079185366 cites W1951259843 @default.
- W2079185366 cites W1970157828 @default.
- W2079185366 cites W1977978576 @default.
- W2079185366 cites W1979032223 @default.
- W2079185366 cites W1994792016 @default.
- W2079185366 cites W1995338261 @default.
- W2079185366 cites W2003405170 @default.
- W2079185366 cites W2006790257 @default.
- W2079185366 cites W2010766264 @default.
- W2079185366 cites W2019490196 @default.
- W2079185366 cites W2022609513 @default.
- W2079185366 cites W2031364568 @default.
- W2079185366 cites W2038062994 @default.
- W2079185366 cites W2049097459 @default.
- W2079185366 cites W2062609628 @default.
- W2079185366 cites W2067015123 @default.
- W2079185366 cites W2072079949 @default.
- W2079185366 cites W2072225623 @default.
- W2079185366 cites W2073474576 @default.
- W2079185366 cites W2074317222 @default.
- W2079185366 cites W2093983103 @default.
- W2079185366 cites W2095925969 @default.
- W2079185366 cites W2096749374 @default.
- W2079185366 cites W2104205504 @default.
- W2079185366 cites W2106917466 @default.
- W2079185366 cites W2110796758 @default.
- W2079185366 cites W2121356084 @default.
- W2079185366 cites W2121857159 @default.
- W2079185366 cites W2123579775 @default.
- W2079185366 cites W2142427836 @default.
- W2079185366 cites W2153316480 @default.
- W2079185366 cites W2158662692 @default.
- W2079185366 cites W2169670265 @default.
- W2079185366 cites W4211060707 @default.
- W2079185366 doi "https://doi.org/10.1371/journal.pone.0064644" @default.
- W2079185366 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3667183" @default.
- W2079185366 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23734212" @default.
- W2079185366 hasPublicationYear "2013" @default.
- W2079185366 type Work @default.
- W2079185366 sameAs 2079185366 @default.
- W2079185366 citedByCount "50" @default.
- W2079185366 countsByYear W20791853662013 @default.
- W2079185366 countsByYear W20791853662014 @default.
- W2079185366 countsByYear W20791853662015 @default.
- W2079185366 countsByYear W20791853662016 @default.
- W2079185366 countsByYear W20791853662017 @default.
- W2079185366 countsByYear W20791853662018 @default.
- W2079185366 countsByYear W20791853662019 @default.
- W2079185366 countsByYear W20791853662020 @default.
- W2079185366 countsByYear W20791853662021 @default.
- W2079185366 countsByYear W20791853662022 @default.
- W2079185366 countsByYear W20791853662023 @default.
- W2079185366 crossrefType "journal-article" @default.
- W2079185366 hasAuthorship W2079185366A5007069708 @default.
- W2079185366 hasAuthorship W2079185366A5019497452 @default.
- W2079185366 hasAuthorship W2079185366A5060358338 @default.
- W2079185366 hasAuthorship W2079185366A5060420474 @default.
- W2079185366 hasAuthorship W2079185366A5082178462 @default.
- W2079185366 hasAuthorship W2079185366A5091893555 @default.
- W2079185366 hasBestOaLocation W20791853661 @default.
- W2079185366 hasConcept C153911025 @default.
- W2079185366 hasConcept C178790620 @default.
- W2079185366 hasConcept C181080969 @default.
- W2079185366 hasConcept C181199279 @default.
- W2079185366 hasConcept C185592680 @default.
- W2079185366 hasConcept C190283241 @default.
- W2079185366 hasConcept C2775934118 @default.
- W2079185366 hasConcept C2777318727 @default.
- W2079185366 hasConcept C2779116991 @default.
- W2079185366 hasConcept C28406088 @default.
- W2079185366 hasConcept C31573885 @default.
- W2079185366 hasConcept C519063684 @default.
- W2079185366 hasConcept C523546767 @default.
- W2079185366 hasConcept C54355233 @default.
- W2079185366 hasConcept C55493867 @default.
- W2079185366 hasConcept C56928146 @default.
- W2079185366 hasConcept C79879829 @default.
- W2079185366 hasConcept C86803240 @default.
- W2079185366 hasConcept C89423630 @default.
- W2079185366 hasConcept C94030615 @default.
- W2079185366 hasConcept C95444343 @default.
- W2079185366 hasConceptScore W2079185366C153911025 @default.
- W2079185366 hasConceptScore W2079185366C178790620 @default.
- W2079185366 hasConceptScore W2079185366C181080969 @default.